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Titolo:
A NEW APPROACH TO DIALYSIS IN SEQUENTIAL INJECTION SYSTEMS - SPECTROPHOTOMETRIC DETERMINATION OF L(-LACTATE IN WINES())
Autore:
ARAUJO AN; LIMA JLFC; SARAIVA MLMFS; ZAGATTO EAG;
Indirizzi:
UNIV OPORTO,FAC PHARM,DEPT PHYS CHEM,CEQUP P-4050 OPORTO PORTUGAL UNIV SAO PAULO,CTR NUCL ENERGY AGR,DEPT ANALYT CHEM PIRACICABA SP BRAZIL
Titolo Testata:
American journal of enology and viticulture
fascicolo: 4, volume: 48, anno: 1997,
pagine: 428 - 432
SICI:
0002-9254(1997)48:4<428:ANATDI>2.0.ZU;2-1
Fonte:
ISI
Lingua:
ENG
Soggetto:
D-LACTATE DEHYDROGENASE; D-LACTIC ACID; ENZYMATIC DETERMINATION;
Keywords:
SEQUENTIAL INJECTION ANALYSIS (SIA); L(+)-LACTATE; WINE;
Tipo documento:
Article
Natura:
Periodico
Citazioni:
19
Recensione:
Indirizzi per estratti:
Citazione:
A.N. Araujo et al., "A NEW APPROACH TO DIALYSIS IN SEQUENTIAL INJECTION SYSTEMS - SPECTROPHOTOMETRIC DETERMINATION OF L(-LACTATE IN WINES())", American journal of enology and viticulture, 48(4), 1997, pp. 428-432

Abstract

A sequential injection analysis (SIA) system for the spectrophotometric determination of L(+)-lactate using lactate oxidase/peroxidase was developed and applied to wine analysis. It incorporates a dialysis unit between the pump and the selecting valve to provide on-line dilutionand interference minimization of the sample matrix. A volume of 100 mu L of carrier solution trapped in one channel of the dialysis unit, acts as donor and acceptor solution. With an assay cycle of 262 seconds, the system allows a sample throughput of 14 samples per hour and yields precise results (rsd < 2%). Peroxidase (0.0512 IU) and 0.0384 IU of lactate oxidase promote the conversion of L(+)-lactate in 160 mu L of sample, allowing its determination within the 0.25 to 2.5 g/L range. A detection limit of 0.074 g/L was calculated. Results are in good agreement with those obtained with the conventional method (Boehringer UV-Kit).

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Documento generato il 26/11/20 alle ore 19:17:04