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Titolo:
THE CYTOSKELETAL INTERMEDIATE FILAMENTS OF CANINE RETINAL-PIGMENT EPITHELIAL-CELLS IN-VIVO AND IN-VITRO
Autore:
MCLELLAN GJ; BEDFORD PGC;
Indirizzi:
ROYAL VET COLL,DEPT SMALL ANIM MED & SURG,HAWKSHEAD LANE HATFIELD AL97TA HERTS ENGLAND
Titolo Testata:
Research in Veterinary Science
fascicolo: 3, volume: 63, anno: 1997,
pagine: 245 - 251
SICI:
0034-5288(1997)63:3<245:TCIFOC>2.0.ZU;2-8
Fonte:
ISI
Lingua:
ENG
Soggetto:
IMMUNOHISTOCHEMICAL EVALUATION; MONOCLONAL-ANTIBODIES; HUMAN-EYE; VIMENTIN; EXPRESSION; PROTEINS; CYTOKERATIN; TUMORS; PATTERNS; CULTURE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
33
Recensione:
Indirizzi per estratti:
Citazione:
G.J. Mclellan e P.G.C. Bedford, "THE CYTOSKELETAL INTERMEDIATE FILAMENTS OF CANINE RETINAL-PIGMENT EPITHELIAL-CELLS IN-VIVO AND IN-VITRO", Research in Veterinary Science, 63(3), 1997, pp. 245-251

Abstract

The cytoskeletal intermediate filament characteristics of normal, freshly isolated and subcultured canine retinal pigment epithelial (RPE) cells were studied using immunocytochemistry and immunoblotting techniques. Commercially available primary antibodies recognising a broad range of cytokeratins and vimentin were selected. Cytokeratin reactivitywas a constant feature of all canine RPE cells. The main cytokeratinsexpressed by cultured RPE cells included 8, 18 and 19. This finding is consistent with the published findings of work carried out in other mammalian species including man. Freshly isolated RPE cells stained positively with broad-spectrum antibodies to cytokeratins but generally did not stain with antibodies specific to cytokeratins 18 or 19 and did not stain with antibodies to vimentin, or stained only very weakly. After a short time in culture however, cells demonstrated intense positive staining for vimentin. This study demonstrated that cytokeratin immunoreactivity (in conjunction with vimentin immunoreactivity in vitro) is a useful and consistent marker for canine RPE cells.

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Documento generato il 18/09/20 alle ore 20:01:16