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Titolo:
NITRIC-OXIDE INHIBITS OCT-1 DNA-BINDING ACTIVITY IN CULTURED VASCULARSMOOTH-MUSCLE CELLS
Autore:
LIU XK; ABERNETHY DR; ANDRAWIS NS;
Indirizzi:
GEORGETOWN UNIV,MED CTR,DEPT PHARMACOL,DIV CLIN PHARMACOL,BLDG D,ROOM393,3900 RESERVOIR RD NW WASHINGTON DC 20007 GEORGETOWN UNIV,MED CTR,DEPT PHARMACOL,DIV CLIN PHARMACOL WASHINGTON DC 20007
Titolo Testata:
Life sciences
fascicolo: 8, volume: 62, anno: 1998,
pagine: 739 - 749
SICI:
0024-3205(1998)62:8<739:NIODAI>2.0.ZU;2-D
Fonte:
ISI
Lingua:
ENG
Soggetto:
GENERATING VASODILATORS; GUANYLATE-CYCLASE; ORGANIC NITRATES; ANGIOTENSIN-II; POU DOMAIN; TRANSCRIPTION; PROLIFERATION; MITOGENESIS; REPLICATION; NITROSOTHIOLS;
Keywords:
NITRIC OXIDE; OCT-1; VASCULAR SMOOTH MUSCLE CELL; DNA BINDING ACTIVITY;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
32
Recensione:
Indirizzi per estratti:
Citazione:
X.K. Liu et al., "NITRIC-OXIDE INHIBITS OCT-1 DNA-BINDING ACTIVITY IN CULTURED VASCULARSMOOTH-MUSCLE CELLS", Life sciences, 62(8), 1998, pp. 739-749

Abstract

Since Oct-1 is a ubiquitous DNA binding protein shown to play an important role in regulating cell proliferation and possess structural characteristics consistent with a nitric oxide (NO) target, we studied NOregulation of the DNA binding activity of Oct-1 in the A7R5 vascular smooth muscle cell (VSMC) line. Two NO donors, sodium nitroprusside (SNP) and S-nitrosoacetyl-penicillamine (SNAP) were directly added to the nuclear extract-oligonucleotide reaction mixture, respectively and the effect on Oct-1 DNA binding activity was evaluated by gel shift assay. Both NO donors (0.01-1 mM) inhibited the DNA binding activity of Oct-1. This inhibitory effect was not attenuated by dithiothreitol (DTT) (1 mM) while in contrast, DTT did antagonize the effect of diamide on Oct-1 DNA binding activity. The NO effect on Oct-1 has some specificity; as the NO donors had no effect on myc DNA binding activity. The inhibitory effect of NO donors was reproduced in A7R5 cells, without affecting their viability. These findings provide the first evidence that NO inhibits the DNA binding activity of Oct-1, probably through a cGMP independent mechanism and suggests that NO may inhibit mitogenesis in part through an effect on Oct-1 DNA binding activity in VSMCs.

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Documento generato il 12/07/20 alle ore 04:40:15