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Titolo:
INTRACELLULAR MELATONIN DISTRIBUTION IN CULTURED-CELL LINES
Autore:
FINOCCHIARO LME; GLIKIN GC;
Indirizzi:
CONSEJO NACL INVEST CIENT & TECN,INGEBI,INST INVEST INGN GENET & BIOLMOL,VTA OBLIGADO 2490 RA-1428 BUENOS AIRES DF ARGENTINA UNIV BUENOS AIRES,FAC CIENCIAS EXACTAS & NAT RA-1428 BUENOS AIRES DF ARGENTINA
Titolo Testata:
Journal of pineal research
fascicolo: 1, volume: 24, anno: 1998,
pagine: 22 - 34
SICI:
0742-3098(1998)24:1<22:IMDICL>2.0.ZU;2-4
Fonte:
ISI
Lingua:
ENG
Soggetto:
BLOOD MONONUCLEAR LEUKOCYTES; DNA-REPLICATION SITES; MESSENGER-RNA LEVELS; LIPID-PEROXIDATION; NEUROHORMONE MELATONIN; PHOSPHATASE-ACTIVITY; MAMMALIAN-TISSUES; HARDERIAN-GLAND; SYRIAN-HAMSTERS; VISIBLE-LIGHT;
Keywords:
MELATONIN; SEROTONIN; ACTIN; BROMODEOXYURIDINE; IMMUNOFLUOR-ESCENCE; 3T3 MOUSE FIBROBLASTS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
58
Recensione:
Indirizzi per estratti:
Citazione:
L.M.E. Finocchiaro e G.C. Glikin, "INTRACELLULAR MELATONIN DISTRIBUTION IN CULTURED-CELL LINES", Journal of pineal research, 24(1), 1998, pp. 22-34

Abstract

A specific antibody combined with a fluorescein-labeled immunoglobulin was used to investigate the topographic distribution of melatonin ina variety of cells of different origins. Positive identification of both nuclear and cytosolic melatonin was confirmed in all the tested cells: Swiss 3T3 mouse fibroblasts, BCG1 bovine granulosa, NB41A3 mouse neuroblastoma, F9 mouse teratocarcinoma, MDCK normal canine kidney derived and human HeLa cell lines, as well as in human peripheral blood mononuclear leukocytes and rat splenic cells. In 3T3 mouse fibroblasts melatonin immunofluorescence partially colocalized with actin and serotonin immunostaining, but not with tubulin or actin stress fibers. Several distinct patterns of subcellular melatonin distribution, different from the bromodeoxyuridine-labeled replication profiles, have been discerned throughout the cell cycle of synchronized 3T3 cells. In addition, synchronized 3T3 mouse fibroblasts cultured in the presence of 10(-3) M melatonin progressed more slowly through the cell cycle than control cells. These results suggest that melatonin may interact directly with nuclear and cytoskeletal structures probably affecting different cell function such as cell cycle control, subcellular organization and genome stability.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 24/09/20 alle ore 22:31:13