Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
KUNITZ-TYPE SOYBEAN TRYPSIN-INHIBITOR REVISITED - REFINED STRUCTURE OF ITS COMPLEX WITH PORCINE TRYPSIN REVEALS AN INSIGHT INTO THE INTERACTION BETWEEN A HOMOLOGOUS INHIBITOR FROM ERYTHRINA-CAFFRA AND TISSUE-TYPE PLASMINOGEN-ACTIVATOR
Autore:
SONG HK; SUH SW;
Indirizzi:
SEOUL NATL UNIV,COLL NAT SCI,DEPT CHEM SEOUL 151742 SOUTH KOREA SEOUL NATL UNIV,COLL NAT SCI,DEPT CHEM SEOUL 151742 SOUTH KOREA SEOUL NATL UNIV,COLL NAT SCI,CTR MOL CATALYSIS SEOUL 151742 SOUTH KOREA
Titolo Testata:
Journal of Molecular Biology
fascicolo: 2, volume: 275, anno: 1998,
pagine: 347 - 363
SICI:
0022-2836(1998)275:2<347:KSTR-R>2.0.ZU;2-Q
Fonte:
ISI
Lingua:
ENG
Soggetto:
FIBROBLAST GROWTH-FACTOR; AMINO-ACID-SEQUENCES; WINGED BEAN-SEEDS; CRYSTAL-STRUCTURE; 3-DIMENSIONAL STRUCTURE; SUBTILISIN INHIBITOR; WEISSENBERG CAMERA; DIFFRACTION DATA; REACTIVE SITE; RESOLUTION;
Keywords:
CRYSTAL STRUCTURE; KUNITZ-TYPE PROTEINASE INHIBITOR; SOYBEAN TRYPSIN INHIBITOR; BETA-TREFOIL FOLD; INHIBITION OF PLASMINOGEN ACTIVATOR;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
57
Recensione:
Indirizzi per estratti:
Citazione:
H.K. Song e S.W. Suh, "KUNITZ-TYPE SOYBEAN TRYPSIN-INHIBITOR REVISITED - REFINED STRUCTURE OF ITS COMPLEX WITH PORCINE TRYPSIN REVEALS AN INSIGHT INTO THE INTERACTION BETWEEN A HOMOLOGOUS INHIBITOR FROM ERYTHRINA-CAFFRA AND TISSUE-TYPE PLASMINOGEN-ACTIVATOR", Journal of Molecular Biology, 275(2), 1998, pp. 347-363

Abstract

The Kunitz-type trypsin inhibitor from soybean (STI) consists of 181 amino acid residues with two disulfide bridges. Its crystal structureshave been determined in complex with porcine pancreatic trypsin in two crystal forms (an orthorhombic form at 1.75 Angstrom resolution and a tetragonal form at 1.9 Angstrom) and in the free state at 2.3 Angstrom resolution. They have been refined to crystallographic R-values of 18.9%, 21.6% and 19.8%, respectively. The three models of STI reportedhere represent a significant improvement over the partial inhibitor structure in the complex, which was previously determined at a nominal resolution of 2.6 Angstrom by the multiple isomorphous replacement method. This study provides the first high-resolution picture of the complex between a Kunitz-type proteinase inhibitor with its cognate proteinase. Many of the external loops of STI show high B-factors, both in the free and the complexed states, except the reactive site loop whose B-factors are dramatically reduced upon complexation. The reactive site loop of STI adopts a canonical conformation similar to those in other substrate-like inhibitors. The P1 carbonyl group displays no out-of-plane displacement and thus retains a nominal trigonal planar geometry. Modeling studies on the complex between a homologous Kunitz-type trypsin inhibitor DE-3 from Erythrina caffra and the human tissue-type plasminogen activator reveal a new insight into the specific interactions which could play a crucial role in their binding. (C) 1998 Academic Press Limited.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 01/12/20 alle ore 07:07:31