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Titolo:
RETENTION AND REGENERATION OF NATIVE NAD(H) IN NONCHARGED ULTRAFILTRATION MEMBRANE REACTORS - APPLICATION TO L-LACTATE AND GLUCONATE PRODUCTION
Autore:
OBON JM; MANJON A; IBORRA JL;
Indirizzi:
UNIV MURCIA,FAC QUIM,DEPT BIOQUIM & BIOL MOL & INMUNOL B,APDO CORREOS4021 E-30001 MURCIA SPAIN UNIV MURCIA,FAC QUIM,DEPT BIOQUIM & BIOL MOL & INMUNOL B E-30001 MURCIA SPAIN
Titolo Testata:
Biotechnology and bioengineering
fascicolo: 5, volume: 57, anno: 1998,
pagine: 510 - 517
SICI:
0006-3592(1998)57:5<510:RARONN>2.0.ZU;2-U
Fonte:
ISI
Lingua:
ENG
Soggetto:
COENZYME REGENERATION; GLUCOSE-DEHYDROGENASE; SORBITOL PRODUCTION; BIOREACTOR; NADP(H); SYSTEM;
Keywords:
NAD(H) RETENTION; COENZYME REGENERATION; COUPLED REACTIONS; GLUCOSE DEHYDROGENASE; LACTATE DEHYDROGENASE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
26
Recensione:
Indirizzi per estratti:
Citazione:
J.M. Obon et al., "RETENTION AND REGENERATION OF NATIVE NAD(H) IN NONCHARGED ULTRAFILTRATION MEMBRANE REACTORS - APPLICATION TO L-LACTATE AND GLUCONATE PRODUCTION", Biotechnology and bioengineering, 57(5), 1998, pp. 510-517

Abstract

NAD(H) was retained in a noncharged ultrafiltration membrane reactor for the simultaneous and continuous production of L-lactate and gluconate with coenzyme regeneration. Polyethyleneimine (PEI), a 50-kDa cationic polymer, achieved coenzyme retentions above 0.8 for PEI/NAD(H) molar ratios higher than 5. The ionic strength of the inlet medium caused a decrease of NAD(H) retention that can be counterbalanced by an initial addition of 1% bovine serum albumin (BSA). Continuous reactor performance in the presence of PEI and BSA showed that NAD(H), glucose dehydrogenase, and lactate dehydrogenase were retained by 10-kDa ultrafiltration membranes; L-lactate and gluconate were produced at conversions higher than 95%. PEI enhanced the thermal stability of the enzymes used and increased the catalytic efficiency of glucose dehydrogenase, while no effect was found on the kinetic parameters of lactate dehydrogenase. A model that implements the kinetic equations of the two enzymes describes the reactor behavior satisfactorily. In brief, the use ofPEI to retain NAD(H) is a new interesting approach to be widely applied in continuous synthesis with the large number of known dehydrogenases. (C) 1998 John Wiley & Sons, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 21/09/20 alle ore 05:41:58