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Titolo:
CHANGES IN PROTEIN-KINASE-C IN EARLY CARDIOMYOPATHY AND IN GRACILIS MUSCLE IN THE BB WOR DIABETIC RAT/
Autore:
GILES TD; OUYANG J; KERUT EK; GIVEN MB; ALLEN GE; MCILWAIN EF; GREENBERG SS;
Indirizzi:
LOUISIANA STATE UNIV,MED CTR,DEPT MED,CARDIOL SECT,CARDIOVASC RES LABS,1542 TULANE AVE,RM 334 NEW ORLEANS LA 70112 LOUISIANA STATE UNIV,MED CTR,ALCOHOL RES CTR,DEPT PHYSIOL NEW ORLEANSLA 70112
Titolo Testata:
American journal of physiology. Heart and circulatory physiology
fascicolo: 1, volume: 43, anno: 1998,
pagine: 295 - 307
SICI:
0363-6135(1998)43:1<295:CIPIEC>2.0.ZU;2-Z
Fonte:
ISI
Lingua:
ENG
Soggetto:
OXIDE SYNTHASE-II; DIASTOLIC FUNCTION; ACTOMYOSIN MGATPASE; CARDIAC MYOCYTES; GLYCEMIC CONTROL; MESSENGER-RNA; IN-VIVO; HEART; INSULIN; INHIBITION;
Keywords:
ECHOCARDIOGRAPHY; GENETIC DIABETES; DOPPLER FLOWMETRY; PROTEIN KINASE C ISOZYMES; MYOCARDIAL CONTRACTILITY;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
44
Recensione:
Indirizzi per estratti:
Citazione:
T.D. Giles et al., "CHANGES IN PROTEIN-KINASE-C IN EARLY CARDIOMYOPATHY AND IN GRACILIS MUSCLE IN THE BB WOR DIABETIC RAT/", American journal of physiology. Heart and circulatory physiology, 43(1), 1998, pp. 295-307

Abstract

Hyperglycemia can upregulate protein kinase C (PKC), which may be an important mediator of the progression from normal heart and muscle function to diabetic myopathy in the myocardium and skel- etal muscle in type 1 insulin-dependent diabetes mellitus (IDM). We evaluated this possibility during the early stage of IDM in BB/Wor diabetic (D) rats and age-matched BB/Wor diabetes-resistant (DR) rats. Interventricular septal thickness, E wave peak velocity of tricuspid inflow (both minimumand maximum), and left ventricular (LV) weight index were increased, and the rate of change in LV pressure (LV dP/dt) decreased in D rats subjected to M-mode and two-dimensional echocardiography and hemodynamic recording of heart rate, LV pressure (LVP), +LV dP/dt, -LV dP/dt, and LV end-diastolic pressure (LVEDP) in vivo and in vitro 41 days afterthe onset of hyperglycemia. Whole ventricle basal PKC activity was increased by 44.4 and 18.4% in the particulate and soluble fractions, respectively, from D rats compared with that from DR rats using r-P-32 phosphorylation of appropriate peptide substrates. When measured by Western blot gel densitometry, particulate PKC-alpha and PKC-delta content increased by 89 and 24%, respectively, but soluble PKC-beta and soluble and particulate PKC-epsilon were unchanged compared with that of DR rats. Similarly, gracilis muscle PKC activity and PKC-alpha and PKC-delta were elevated in the gracilis muscle, whereas that of the circulating neutrophil did not differ between the D and DR rats. Thus, in vivo, the early diabetic cardiomyopathy of the D rat is characterized bya restrictive LV with increased septal thickness and is associated with elevated PKC activity and increased amounts of myocardial particulate PKC-alpha and PKC-delta, which are also seen in the skeletal muscle. We conclude that increased PKC isozymes may play a pivotal role during IDM in the development of diabetic cardiomyopathy and skeletal muscle myopathy.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 02/07/20 alle ore 22:10:04