Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
THE EFFECT OF MANNAN-BINDING LECTIN ON OPSONOPHAGOCYTOSIS OF NEISSERIA-MENINGITIDIS
Autore:
DROGARIAPIRANTHITOU M; FIJEN CAP; THIEL S; PLATONOV A; JENSEN L; DANKERT J; KUIJPER EJ;
Indirizzi:
UNIV AMSTERDAM,DEPT MED MICROBIOL,MEIBERGDREEF 15 NL-1105 AZ AMSTERDAM NETHERLANDS AARHUS UNIV,INST MED MICROBIOL DK-8000 AARHUS DENMARK INST EPIDEMIOL MOSCOW 111123 RUSSIA
Titolo Testata:
Immunopharmacology
fascicolo: 1-2, volume: 38, anno: 1997,
pagine: 93 - 99
SICI:
0162-3109(1997)38:1-2<93:TEOMLO>2.0.ZU;2-E
Fonte:
ISI
Lingua:
ENG
Soggetto:
SYSTEMIC LUPUS-ERYTHEMATOSUS; MENINGOCOCCAL DISEASE; PROTEIN; COMPLEMENT;
Keywords:
MANNAN-BINDING LECTIN; OPSONOPHAGOCYTOSIS; NEISSERIA MENINGITIDIS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
19
Recensione:
Indirizzi per estratti:
Citazione:
M. Drogariapiranthitou et al., "THE EFFECT OF MANNAN-BINDING LECTIN ON OPSONOPHAGOCYTOSIS OF NEISSERIA-MENINGITIDIS", Immunopharmacology, 38(1-2), 1997, pp. 93-99

Abstract

Mannan-binding lectin (MEL), an acute phase protein with a structure and a function very similar to that of Clq, is known to act as an opsonin binding to a number of microorganisms. In order to investigate theeffect of MBL on the phagocytic killing of meningococci, a serogroup B meningococcal strain (H44/76) and its unencapsulated variant v24, aswell as a serogroup A meningococcal strain were opsonized with MBL (purified from normal human plasma at the State Serum Institute, Denmark) and used in a phagocytic killing assay at a density of 7 x 10(3) CFU/ml. Polymorphonuclear cells (PMNs) from one healthy donor were isolated by density gradient centrifugation over Percoll and added to the system (7 x 10(6) cells/ml). In a first set of experiments without addition of serum or complement, no influence of MBL was observed on the killing of any of these strains. Addition of MBL to non-opsonized bacteria of the serogroup A strain did not result in enhanced killing either; on the contrary, the growth of this strain increased significantly when a high MBL concentration (40 mu g/ml) was used in the presence of PMNs. Further investigations were performed using sera of five individuals with late complement component deficiency (LCCD) and a concomitant MBL deficiency, vaccinated with a tetra-valent (ACYW135) meningococcal capsular polysaccharide vaccine. Pre-and post-vaccination sera (50%final concentration) were tested against a group A strain opsonized or not with MEL. In only one patient was there a moderate increase of killing of the opsonized bacteria after vaccination compared to pre-vaccination serum. Our results suggest that MBL may not play a significant role in the opsonophagocytosis of meningococci, irrespective of its binding to unencapsulated and serogroup A strains. (C) 1997 Elsevier Science B.V.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/09/20 alle ore 03:33:14