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Titolo: A UNIVERSAL RADIOCHEMICAL HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC ASSAY FOR THE DETERMINATION OF UDP-GLUCURONOSYLTRANSFERASE ACTIVITY
Autore: ETHELL BT; ANDERSON GD; BEAUMONT K; RANCE DJ; BURCHELL B;
- Indirizzi:
- NINEWELLS HOSP & MED SCH,DEPT MOL & CELLULAR PATHOL DUNDEE DD1 9SY SCOTLAND NINEWELLS HOSP & MED SCH,DEPT MOL & CELLULAR PATHOL DUNDEE DD1 9SY SCOTLAND UNIV WASHINGTON,SCH PHARM SEATTLE WA 98195 PFIZER LTD,DEPT PHARMACEUT RES & DEV SANDWICH CT13 9NJ KENT ENGLAND
- Titolo Testata:
- Analytical biochemistry
fascicolo: 1,
volume: 255,
anno: 1998,
pagine: 142 - 147
- SICI:
- 0003-2697(1998)255:1<142:AURHLA>2.0.ZU;2-X
- Fonte:
- ISI
- Lingua:
- ENG
- Soggetto:
- XENOBIOTIC GLUCURONIDATION; ARYLCARBOXYLIC ACIDS; STABLE EXPRESSION; LIVER-MICROSOMES; BILIRUBIN; ETHINYLESTRADIOL; SPECIFICITY; FAMILY;
- Tipo documento:
- Article
- Natura:
- Periodico
- Settore Disciplinare:
- Science Citation Index Expanded
- Science Citation Index Expanded
- Science Citation Index Expanded
- Citazioni:
- 24
- Recensione:
- Indirizzi per estratti:
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- Citazione:
- B.T. Ethell et al., "A UNIVERSAL RADIOCHEMICAL HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC ASSAY FOR THE DETERMINATION OF UDP-GLUCURONOSYLTRANSFERASE ACTIVITY", Analytical biochemistry, 255(1), 1998, pp. 142-147
Abstract
A new unified assay for the determination of UDP-glucuronosyltransferase (UGT) activities has been developed. The resolution of [C-14]uridine diphosphate glucuronic acid from radiolabeled glucuronides formed by incorporation of this radiolabel can now be achieved by a sensitive and rapid-gradient HPLC method which utilizes a radioactivity endpointas a universal detection method. One important application of this method is the determination of kinetic parameters for cloned and expressed UGT isoforms with greater speed and precision than can be afforded by TLC methodology. Moreover, assays with C-14-labeled substrates indicate that gradient HPLC can easily resolve the substrate from the glucuronide products and present an alternative to the time-consuming optimization of conditions for organic phase extraction assays. (C) 1998 Academic Press.
ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/12/20 alle ore 16:27:00