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Titolo:
EXPRESSION OF NADPH OXIDASE IS INDUCED BY ALL-TRANS-RETINOIC ACID BUTNOT BY PHORBOL-MYRISTATE ACETATE AND 1,25-DIHYDROXYVITAMIN-D3 IN THE HUMAN PROMYELOCYTIC CELL-LINE NB4
Autore:
NDIAYE EN; VAISSIERE C; GONZALEZCHRISTEN J; GREGOIRE C; LECABEC V; MARIDONNEAUPARINI I;
Indirizzi:
INST PHARMACOL & BIOL STRUCT,CNRS,UPR 9062,205 ROUTE NARBONNE F-31077TOULOUSE FRANCE INST PHARMACOL & BIOL STRUCT,CNRS,UPR 9062 F-31077 TOULOUSE FRANCE
Titolo Testata:
Leukemia
fascicolo: 12, volume: 11, anno: 1997,
pagine: 2131 - 2136
SICI:
0887-6924(1997)11:12<2131:EONOII>2.0.ZU;2-4
Fonte:
ISI
Lingua:
ENG
Soggetto:
LEUKEMIA-CELLS; MONOCYTIC DIFFERENTIATION; HUMAN NEUTROPHILS; RESPIRATORY BURST; GENE-EXPRESSION; BINDING; MODULATION; ACTIVATION; MEMBRANE; PROTEINS;
Keywords:
NB4 CELLS; NEUTROPHIL; MONOCYTE; DIFFERENTIATION; NADPH OXIDASE; LEUKEMIA;
Tipo documento:
Article
Natura:
Periodico
Citazioni:
29
Recensione:
Indirizzi per estratti:
Citazione:
E.N. Ndiaye et al., "EXPRESSION OF NADPH OXIDASE IS INDUCED BY ALL-TRANS-RETINOIC ACID BUTNOT BY PHORBOL-MYRISTATE ACETATE AND 1,25-DIHYDROXYVITAMIN-D3 IN THE HUMAN PROMYELOCYTIC CELL-LINE NB4", Leukemia, 11(12), 1997, pp. 2131-2136

Abstract

Human promyelocytic cells, NB4, differentiate into neutrophils in response to all-trans retinoic acid (ATRA), It has recently been proposedthat NB4 cells have bilineage potential because these cells are also able to differentiate into monocyte/macrophages when exposed to a combination of 1,25-dihydroxyvitamin D3 (VD3) and phorbol myristate acetate (PMA). Differentiation of myeloid cells into neutrophils or monocytes is associated with the acquisition of the O-2(-) producing enzyme, NADPH oxidase, which plays a critical role in microbial killing. In this study, the expression of the components of the NADPH oxidase complexduring the differentiation of NB4 cells into neutrophils or macrophages has been investigated. Whereas cells exposed to ATRA were able to produce O-2(-) after 2 days of differentiation, they remain unable to generate O-2(-) when exposed to PMA or PMA + VD3, With the exception ofp21rac, none of the other oxidase components was expressed in non-differentiated cells, Addition of ATRA induced the progressive expressionand accumulation of p22phox, p91phox, p47phox and p67phox, Compared to the other components, p67phox was expressed late and its expression appeared to correlate most closely with the generation of O-2(-) in the differentiation process, In PMA or PMA + VD3-differentiaied NB4 cells, expression of the NADPH oxidase components was incomplete. Therefore, ATRA induced the expression of a functional NADPH oxidase complex in neutrophil-like NB4 cells, In contrast, when NB4 cells are exposed to monocytic differentiating agents, they acquire only part of the phenotypic characteristics of monocytes and lack one of the major phagocytic functionalities, the respiratory burst oxidase.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 27/11/20 alle ore 23:49:04