Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
INDUCTION OF DNA-REPLICATION IN GERMINAL VESICLES AND IN NUCLEI FORMED IN MATURING MOUSE OOCYTES BY 6-DMAP TREATMENT
Autore:
FULKA J; FIRST NL; LEE C; FULKA J; MOOR RM;
Indirizzi:
INST ANIM PROD CS-10401 PRAGUE 10 CZECH REPUBLIC
Titolo Testata:
Zygote
fascicolo: 3, volume: 5, anno: 1997,
pagine: 213 - 217
SICI:
0967-1994(1997)5:3<213:IODIGV>2.0.ZU;2-Q
Fonte:
ISI
Lingua:
ENG
Soggetto:
MATURATION-PROMOTING FACTOR; 6-DIMETHYLAMINOPURINE 6-DMAP; PROTEIN-KINASES; BOVINE OOCYTES; CELL-CYCLE; TRANSITION; INHIBITION; METAPHASE; BLOCK; BREAKDOWN;
Keywords:
6-DMAP; DNA REPLICATION; OOCYTES;
Tipo documento:
Article
Natura:
Periodico
Citazioni:
22
Recensione:
Indirizzi per estratti:
Citazione:
J. Fulka et al., "INDUCTION OF DNA-REPLICATION IN GERMINAL VESICLES AND IN NUCLEI FORMED IN MATURING MOUSE OOCYTES BY 6-DMAP TREATMENT", Zygote, 5(3), 1997, pp. 213-217

Abstract

Immature mouse oocytes (germinal vesicle stage, GV), oocytes at different stages during maturation (prometaphase to anaphase I) and maturedoocytes (metaphase II arrested) were cultured in 6-dimethylaminopurine (6-DMAP)-supplemented medium also containing bromodeoxyuridine for the assessment of DNA replication in these cells. Immature oocytes remained arrested at the GV stage and DNA replication was never detected in them. On the other hand, oocytes at the prometaphase to anaphase-telophase I stages responded to 6-DMAP treatment by forming nuclei which synthesised DNA. Mature (metaphase II) oocytes did not respond to 6-DMAP and their chromatin remained condensed. DNA synthesis could even beinduced in GV-staged oocytes, but only when they were fused to freshly activated oocytes and incubated in 6-DMAP-supplemented medium.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/11/20 alle ore 04:52:10