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Titolo:
IN-VITRO ANGIOGENESIS IS MODULATED BY THE MECHANICAL PROPERTIES OF FIBRIN GELS AND IS RELATED TO ALPHA(V)BETA(3) INTEGRIN LOCALIZATION
Autore:
VAILHE B; RONOT X; TRACQUI P; USSON Y; TRANQUI L;
Indirizzi:
UNIV GRENOBLE 1,BIOENERGET FONDAMENT & APPL LAB,BP 53 F-38041 GRENOBLE 9 FRANCE UNIV GRENOBLE 1,BIOENERGET FONDAMENT & APPL LAB F-38041 GRENOBLE 9 FRANCE UNIV JOSEPH FOURIER,INST ALBERT BONNIOT,DYNAMIQUE ORG GENOMES LAB F-38706 LA TRONCHE FRANCE FAC MED LA TRONCHE,INST ALBERT BONNIOT,LAB TIMC,UMR CNRS 5525 F-38700LA TRONCHE FRANCE EPHE,LAB NEUROBIOL DEV F-34095 MONTPELLIER 05 FRANCE
Titolo Testata:
In vitro cellular & developmental biology. Animal
fascicolo: 10, volume: 33, anno: 1997,
pagine: 763 - 773
SICI:
1071-2690(1997)33:10<763:IAIMBT>2.0.ZU;2-P
Fonte:
ISI
Lingua:
ENG
Soggetto:
HUMAN-ENDOTHELIAL CELLS; EXTRACELLULAR-MATRIX; CELLULAR TENSEGRITY; FOCAL CONTACTS; FIBRONECTIN; ADHESION; INVITRO; COLLAGEN; CYTOSKELETON; SUBSTRATUM;
Keywords:
ENDOTHELIAL CELL; NEOVASCULARIZATION IN-VITRO; FIBRIN; MATRIX MECHANICAL PROPERTIES; DYNAMIC ANALYSIS; ALPHA(V)BETA(3) INTEGRINS;
Tipo documento:
Article
Natura:
Periodico
Citazioni:
47
Recensione:
Indirizzi per estratti:
Citazione:
B. Vailhe et al., "IN-VITRO ANGIOGENESIS IS MODULATED BY THE MECHANICAL PROPERTIES OF FIBRIN GELS AND IS RELATED TO ALPHA(V)BETA(3) INTEGRIN LOCALIZATION", In vitro cellular & developmental biology. Animal, 33(10), 1997, pp. 763-773

Abstract

This study deals with the role of the mechanical properties of matrices in in vitro angiogenesis. The ability of rigid fibrinogen matrices with fibrin gels to promote capillarylike structures was compared. Therole of the mechanical properties of the fibrin gels was assessed by varying concentration of the fibrin gels. When the concentration of fibrin gels was decreased from 2 mg/ml to 0.5 mg/ml, the capillarylike network increased. On rigid fibrinogen matrices, capillarylike structures were not formed. The extent of the capillarylike network formed on fibrin gels having the lowest concentration depended on the number of cells seeded. The dynamic analysis of capillarylike network formation permitted a direct visualization of a progressive stretching of the 0.5 mg/ml fibrin gels. This stretching was not observed when fibrin concentration increases. This analysis shows that 10 h after seeding, a prearrangement of cells into ringlike structures was observed. These ringlike structures grew in size. Between 16 and 24 h after seeding, the capillarylike structures were formed at the junction to two ringlike structures. Analysis of the alpha(v)beta(3) integrin localization demonstrates that cell adhesion to fibrinogen is mediated through the alpha(v)beta(3) integrin localized into adhesion plaques. Conversely, cell adhesion to fibrin shows a diffuse and dot-contact distribution. We suggest that the balance of the stresses between the tractions exerted by the cells and the resistance of the fibrin gels triggers an angiogenic signal into the intracellular compartment. This signal could be associated with modification in the alpha(v)beta(3) integrin distribution.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 10/07/20 alle ore 14:30:21