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Titolo:
TRANSGENIC CUCUMBER PLANTS HARBORING A RICE CHITINASE GENE EXHIBIT ENHANCED RESISTANCE TO GRAY MOLD (BOTRYTIS-CINEREA)
Autore:
TABEI Y; KITADE S; NISHIZAWA Y; KIKUCHI N; KAYANO T; HIBI T; AKUTSU K;
Indirizzi:
NATL INST AGROBIOL RESOURCES TSUKUBA IBARAKI 305 JAPAN IBARAKI UNIV,FAC AGR AMIMACHI IBARAKI 30003 JAPAN UNIV TOKYO,FAC AGR,BUNKYO KU TOKYO 113 JAPAN
Titolo Testata:
Plant cell reports
fascicolo: 3, volume: 17, anno: 1998,
pagine: 159 - 164
SICI:
0721-7714(1998)17:3<159:TCPHAR>2.0.ZU;2-6
Fonte:
ISI
Lingua:
ENG
Soggetto:
PATHOGENESIS-RELATED PROTEINS; DISEASE RESISTANCE; AGROBACTERIUM-TUMEFACIENS; SATIVUS L; EXPRESSION; TRANSFORMATION; TOMATO; REGENERATION; TOBACCO; CLONING;
Keywords:
CUCUMBER; TRANSFORMATION; AGROBACTERIUM TUMEFACIENS; CHITINASE; BOTRYTIS CINEREA; DISEASE RESISTANCE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
23
Recensione:
Indirizzi per estratti:
Citazione:
Y. Tabei et al., "TRANSGENIC CUCUMBER PLANTS HARBORING A RICE CHITINASE GENE EXHIBIT ENHANCED RESISTANCE TO GRAY MOLD (BOTRYTIS-CINEREA)", Plant cell reports, 17(3), 1998, pp. 159-164

Abstract

A rice chitinase cDNA (RCC2) driven by the CaMV 35S promoter was introduced into cucumber (Cucumis sativus L.) through Agrobacterium mediation. More than 200 putative transgenic shoots were regenerated and grown on MS medium supplemented with 100 mg/l kanamycin. Sixty elongated shoots were examined for the presence of the integrated RCC2 gene and subsequently confirmed to have it. Of these, 20 were tested for resistance against gray mold (Botrytis cinerea) by infection with the conidia: 15 strains out of the 20 independent shoots exhibited a higher resistance than the control (non-transgenic plants). Three transgenic cucumber strains (designated CR29, CR32 and CR33) showed the highest resistance against B. cinerea: the spread of disease was inhibited completely in these strains. Chitinase gene expression in highly resistant transgenic strains (CR32 and CR33) was compared to that of a susceptible transgenic strain (CR20) and a control. Different responses for disease resistance were observed among the highly resistant strains. CR33 inhibited appressoria formation and penetration of hyphae. Although CR32permitted penetration of hyphae, invasion of the infection hyphae wasrestricted. Furthermore, progenies of CR32 showed a segregation ratioof 3:1 (resistant:susceptible). As the disease resistance against gray mold was confirmed to be inheritable, these highly resistant transgenic cucumber strains would serve as good breeding materials for disease resistance.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 07/07/20 alle ore 21:59:36