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Titolo:
SYNAPTIC CLUSTERING OF GABA(C) RECEPTOR RHO-SUBUNITS IN THE RAT RETINA
Autore:
KOULEN P; BRANDSTATTER JH; ENZ R; BORMANN J; WASSLE H;
Indirizzi:
MAX PLANCK INST HIRNFORSCH,DEUTSCHORDENSTR 46 D-60528 FRANKFURT GERMANY MAX PLANCK INST HIRNFORSCH D-60528 FRANKFURT GERMANY
Titolo Testata:
European journal of neuroscience
fascicolo: 1, volume: 10, anno: 1998,
pagine: 115 - 127
SICI:
0953-816X(1998)10:1<115:SCOGRR>2.0.ZU;2-0
Fonte:
ISI
Lingua:
ENG
Soggetto:
CONE BIPOLAR CELLS; IN-SITU HYBRIDIZATION; OUTER PLEXIFORM LAYER; DRUG-BINDING PROFILE; GLYCINE-RECEPTOR; A-RECEPTOR; MAMMALIAN RETINA; BENZODIAZEPINE RECEPTORS; MONOCLONAL-ANTIBODIES; C-RECEPTOR;
Keywords:
BIPOLAR CELLS; CONVENTIONAL SYNAPSES; GLYCINE RECEPTORS; GABA(A) RECEPTORS; GABA(C) RECEPTOR; RHO-SUBUNIT; RIBBON SYNAPSES; SYNAPTIC CLEFT; SYNAPTIC CLUSTERING;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
83
Recensione:
Indirizzi per estratti:
Citazione:
P. Koulen et al., "SYNAPTIC CLUSTERING OF GABA(C) RECEPTOR RHO-SUBUNITS IN THE RAT RETINA", European journal of neuroscience, 10(1), 1998, pp. 115-127

Abstract

Polyclonal antibodies which recognize the rho-subunits of the GABA(C)receptor were applied to sections of the rat retina. Strong punctate immunoreactivity was found in the inner plexiform layer (IPL), which was shown by electron microscopy to represent a clustering of the GABA(C) receptors at synaptic sites. During postnatal development diffuse rho-immunoreactivity was first observed at postnatal day P3. Distinct labelling of bipolar cells appeared at P7 and punctate, synaptic labelling was observed at P10. In order to show that the rho-immunoreactive puncta coincide with the axons of bipolar cells, double immunostainings of retinal sections with an antiserum against syntaxin 3 and with the rho-antiserum were performed. The experiments showed that rho-immunoreactive puncta are preferentially located on the axon terminals of rod and cone bipolar cells. In order to determine whether GABA(C) receptor rho-subunits coassemble with GABA(A) receptor subunits, double-labelling experiments were performed with subunit specific antisera, Punctate, putative synaptic clustering was observed with all antisera applied, however, GABA(C) receptor expressing puncta did not coincide with GABA(A) receptor containing puncta. This suggests that there are no synaptic GABA receptors in the retina in which GABA(A) and GABA(C) receptor subunits are coassembled. Similar double-labelling experiments were also performed to find out whether GABA(C) receptors and glycine receptors are colocalized. They were clustered at different synapses. This suggests that synaptic GABA(C) receptors consist of rho-subunits andare not coassembled with GABA(A)- or glycine-receptor subunits.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/07/20 alle ore 22:21:13