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Titolo:
EVIDENCE SUGGESTING THAT NITRIC-OXIDE MEDIATES IRON-INDUCED TOXICITY IN CULTURED PROXIMAL TUBULE CELLS
Autore:
CHEN LG; ZHANG BH; HARRIS DCH;
Indirizzi:
WESTMEAD HOSP,DEPT RENAL MED,UNIV SYDNEY WESTMEAD NSW 2145 AUSTRALIA WESTMEAD HOSP,DEPT RENAL MED,UNIV SYDNEY WESTMEAD NSW 2145 AUSTRALIA WESTMEAD HOSP,STORR LIVER UNIT,UNIV SYDNEY WESTMEAD NSW 2145 AUSTRALIA
Titolo Testata:
American journal of physiology. Renal, fluid and electrolyte physiology
fascicolo: 1, volume: 43, anno: 1998,
pagine: 18 - 25
SICI:
0363-6127(1998)43:1<18:ESTNMI>2.0.ZU;2-V
Fonte:
ISI
Lingua:
ENG
Soggetto:
BACTERIAL LIPOPOLYSACCHARIDE; SYNTHASE ACTIVITY; INJURY; NEPHROTOXICITY; MYOGLOBIN; RADICALS; INVITRO;
Keywords:
IRON; CYTOTOXICITY; LIPID PEROXIDATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
31
Recensione:
Indirizzi per estratti:
Citazione:
L.G. Chen et al., "EVIDENCE SUGGESTING THAT NITRIC-OXIDE MEDIATES IRON-INDUCED TOXICITY IN CULTURED PROXIMAL TUBULE CELLS", American journal of physiology. Renal, fluid and electrolyte physiology, 43(1), 1998, pp. 18-25

Abstract

The potential role of nitric oxide (NO) in iron-induced toxicity was studied in proximal tubule cells in primary culture. NO production (NO2-/NO3-) was significantly increased in iron-treated compared with control cells (3.43 +/- 0.08 vs. 1.56 +/- 0.08 nmol/dish, P < 0.01). NO synthase (NOS) activity was also induced by iron treatment; (16.2 +/- 2.0 vs. 0.4 +/- 0.2 nmol of [H-3]citrulline/mg protein, P < 0.01). L-Arginine, a substrate for NOS, augmented iron-induced PTO production andcell damage [lactate dehydrogenase (LDH) leakage], whereas aminoguanidine, an inhibitor of NOS, reduced iron-induced NO production and LDH leakage. Sodium nitroprusside, an exogenous NO donor, induced LDH leakage in a dose-dependent manner, but no effect on lipid peroxidation [malondialdehyde bis[dimethyl acetal] (MDA) production] was observed. Superoxide dismutase and catalase decreased iron-induced MBA production hut did not affect LDH leakage or NO production. Dimethylpyrroline N-oxide and desferal prevented MDA production, LDH leakage, and NO production. We concluded that NO is one of the mediators of iron-induced toxicity in proximal tubule cells. NO-induced toxicity is not dependent on lipid peroxidation. This may explain the variable effect of different antioxidants on eel damage and lipid peroxidation in iron-induced cytotoxicity.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/03/20 alle ore 23:40:52