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Titolo:
FLOW CYTOMETRIC SORTING AND BIOCHEMICAL-CHARACTERIZATION OF THE LATE ENDOSOMAL RAB7-CONTAINING COMPARTMENT
Autore:
MERESSE S; ANDRE P; MISHAL Z; BARAD M; BRUN N; DESJARDINS M; GORVEL JP;
Indirizzi:
CIML,PARC SCI LUMINY,CASE 906 F-13288 MARSEILLE 09 FRANCE CNRS,INSERM,CTR IMMUNOL MARSEILLE FRANCE CTR SOUTIEN RECH CANC VILLEJUIF FRANCE UNIV MONTREAL,FAC MED,DEPT ANAT QUEBEC CITY PQ CANADA
Titolo Testata:
Electrophoresis
fascicolo: 14, volume: 18, anno: 1997,
pagine: 2682 - 2688
SICI:
0173-0835(1997)18:14<2682:FCSABO>2.0.ZU;2-6
Fonte:
ISI
Lingua:
ENG
Soggetto:
ENDOCYTIC MEMBRANE-FUSION; GTP-BINDING-PROTEINS; TRANS-GOLGI NETWORK; CATHEPSIN-D; RAB GTPASES; LOCALIZATION; TRAFFICKING; RECEPTORS; VESICLES; INVITRO;
Keywords:
RAB7; LATE ENDOSOMES; LYSOSOMES; FLOW CYTOMETRIC SORTING; 2-DIMENSIONAL POLYACRYLAMIDE GELS ELECTROPHORESIS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
36
Recensione:
Indirizzi per estratti:
Citazione:
S. Meresse et al., "FLOW CYTOMETRIC SORTING AND BIOCHEMICAL-CHARACTERIZATION OF THE LATE ENDOSOMAL RAB7-CONTAINING COMPARTMENT", Electrophoresis, 18(14), 1997, pp. 2682-2688

Abstract

Rab7 is a small molecular weight GTPase that is known to be associated with late endocytic compartments. Studies in which wild-type or mutant forms of this protein have been overexpressed in mammalian cells have indicated that rab7 plays a role in controlling membrane transport between late endocytic compartments. However, both the precise site(s)of action and localization of rab7 remain unclear. In the present study, we have used density-gradient centrifugation in combination with anew epitope-specific now cytometric sorting method to isolate rab7-containing vesicles from baby hamster kidney (BHK) cells. Electron-micrographs of sorted elements showed a homogeneous population of vesicles that resembles late endosomes. The polypeptide composition of rab7-containing vesicles was then analyzed by two-dimensional (2-D) gel electrophoresis. Rab7-containing vesicles were enriched in the cation-independent mannose 6-phosphate receptor and especially in the precursor forms of cathepsin D. Taken together, these results show that the rab7-containing vesicles are a component of the endocytic pathway that connects late endosomes and lysosomes and in which precursor forms of lysosomal hydrolases, segregated from their receptor, might be included.

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Documento generato il 01/10/20 alle ore 07:21:56