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Titolo:
TEMPLATE-DIRECTED INCORPORATION OF NUCLEOTIDE MIXTURES USING AZOLE-NUCLEOBASE ANALOGS
Autore:
HOOPS GC; ZHANG PM; JOHNSON WT; PAUL N; BERGSTROM DE; DAVISSON VJ;
Indirizzi:
PURDUE UNIV,DEPT MED CHEM & MOL PHARMACOL,1333 ROBERT HEINE PHARM BLDG W LAFAYETTE IN 47907 PURDUE UNIV,DEPT MED CHEM & MOL PHARMACOL W LAFAYETTE IN 47907 WALTHER CANC INST INDIANAPOLIS IN 46208
Titolo Testata:
Nucleic acids research
fascicolo: 24, volume: 25, anno: 1997,
pagine: 4866 - 4871
SICI:
0305-1048(1997)25:24<4866:TIONMU>2.0.ZU;2-8
Fonte:
ISI
Lingua:
ENG
Soggetto:
AQUATICUS DNA-POLYMERASE; THERMUS-AQUATICUS; 8-HYDROXYGUANINE 7,8-DIHYDRO-8-OXOGUANINE; ESCHERICHIA-COLI; DUPLEX STABILITY; RAS-GENE; OLIGONUCLEOTIDES; DEOXYINOSINE; BASES; HYBRIDIZATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
32
Recensione:
Indirizzi per estratti:
Citazione:
G.C. Hoops et al., "TEMPLATE-DIRECTED INCORPORATION OF NUCLEOTIDE MIXTURES USING AZOLE-NUCLEOBASE ANALOGS", Nucleic acids research, 25(24), 1997, pp. 4866-4871

Abstract

DNA that encodes elements for degenerate replication events by use ofartificial nucleobases offers a versatile approach to manipulating sequences for applications in biotechnology, We have designed a family of artificial nucleobases that are capable of assuming multiple hydrogen bonding orientations through internal bond rotations to provide a means for degenerate molecular recognition, Incorporation of these analogs into a single position of a PCR primer allowed for analysis of their template effects on DNA amplification catalyzed by Thermus aquaticus(Tag) DNA polymerase, All of the nucleobase surrogates have similar shapes but differ by structural alterations that influence their electronic character, These subtle distinctions were able to influence the Tao DNA polymerase dependent incorporation of the four natural deoxyribonucleotides and thus, significantly expand the molecular design possibilities for biochemically functional nucleic acid analogs.

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Documento generato il 12/07/20 alle ore 05:59:07