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Titolo:
SPATIOTEMPORAL DYNAMICS OF INTRACELLULAR CALCIUM IN THE MOUSE EGG INJECTED WITH A SPERMATOZOON
Autore:
NAKANO Y; SHIRAKAWA H; MITSUHASHI N; KUWABARA Y; MIYAZAKI S;
Indirizzi:
TOKYO WOMENS MED COLL,DEPT PHYSIOL,SHINJUKU KU,8-1 KAWADA CHO TOKYO 162 JAPAN TOKYO WOMENS MED COLL,DEPT PHYSIOL,SHINJUKU KU TOKYO 162 JAPAN JUNTENDO UNIV,SCH MED,DEPT OBSTET & GYNECOL,BUNKYO KU TOKYO 113 JAPAN NATL INST PHYSIOL SCI,DEPT MOL PHYSIOL,LAB INTRACELLULAR METAB OKAZAKI AICHI 444 JAPAN
Titolo Testata:
Molecular human reproduction
fascicolo: 12, volume: 3, anno: 1997,
pagine: 1087 - 1093
SICI:
1360-9947(1997)3:12<1087:SDOICI>2.0.ZU;2-C
Fonte:
ISI
Lingua:
ENG
Soggetto:
INTRACYTOPLASMIC SPERM INJECTION; HUMAN OOCYTE ACTIVATION; GOLDEN-HAMSTER EGGS; CA2+ RELEASE; ENDOPLASMIC-RETICULUM; MEIOTIC MATURATION; MAMMALIAN EGGS; OSCILLATIONS; FERTILIZATION;
Keywords:
MOUSE EGG; INTRACYTOPLASMIC SPERM INJECTION; INTRACELLULAR CALCIUM CONCENTRATION; CALCIUM OSCILLATIONS;
Tipo documento:
Article
Natura:
Periodico
Citazioni:
31
Recensione:
Indirizzi per estratti:
Citazione:
Y. Nakano et al., "SPATIOTEMPORAL DYNAMICS OF INTRACELLULAR CALCIUM IN THE MOUSE EGG INJECTED WITH A SPERMATOZOON", Molecular human reproduction, 3(12), 1997, pp. 1087-1093

Abstract

Oscillatory rises in intracellular Ca2+ concentration ([Ca2+](i)) arethe pivotal signal in the fertilization of mammalian eggs. The spatiotemporal dynamics of [Ca2+](i) rises in mouse eggs subjected to intracytoplasmic sperm injection (ICSI) were analysed by Ca2+ imaging and compared with those subjected to in-vitro fertilization (IVF). The firstCa2+ transient occurred 15-30 min after ICSI in most eggs, and was followed by Ca2+ oscillations which lasted for at least 6 h at intervalsof similar to 10 min. The pattern of Ca2+ oscillations, an initial relatively larger Ca2+ transient followed by smaller Ca2+ transients, was similar to that at fertilization. Confocal Ca2+ imaging during earlyCa2+ transients showed that, in fertilized eggs, [Ca2+](i) increased in a wave which started from the sperm attachment site and propagated across the egg cytoplasm. In eggs subjected to ICSI, [Ca2+](i) increased gradually and then a Ca2+ spike was generated when [Ca2+](i) reached a certain level. The [Ca2+](i) rise occurred in the whole egg, associated with neither a wave nor significant heterogeneity between the cortical and central regions. It is suggested that cytosolic factor(s) may leak from the injected spermatozoon, diffuse slowly in the egg cytoplasm, and then cause a synchronous Ca2+ release from intracellular Ca2+ stores.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/11/20 alle ore 00:21:56