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Titolo:
IN-TRAP CLEANUP OF PROTEINS FROM ELECTROSPRAY-IONIZATION USING SOFT SUSTAINED OFF-RESONANCE IRRADIATION WITH FOURIER-TRANSFORM ION-CYCLOTRON RESONANCE MASS-SPECTROMETRY
Autore:
TOLIC LP; BRUCE JE; LEI QP; ANDERSON GA; SMITH RD;
Indirizzi:
PACIFIC NW NATL LAB,ENVIRONM MOL SCI LAB,POB 999 RICHLAND WA 99352 PACIFIC NW NATL LAB,ENVIRONM MOL SCI LAB RICHLAND WA 99352
Titolo Testata:
Analytical chemistry
fascicolo: 2, volume: 70, anno: 1998,
pagine: 405 - 408
SICI:
0003-2700(1998)70:2<405:ICOPFE>2.0.ZU;2-#
Fonte:
ISI
Lingua:
ENG
Soggetto:
OLIGONUCLEOTIDES; ACCUMULATION; ASSOCIATIONS; COMPLEXES; ENZYME;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
28
Recensione:
Indirizzi per estratti:
Citazione:
L.P. Tolic et al., "IN-TRAP CLEANUP OF PROTEINS FROM ELECTROSPRAY-IONIZATION USING SOFT SUSTAINED OFF-RESONANCE IRRADIATION WITH FOURIER-TRANSFORM ION-CYCLOTRON RESONANCE MASS-SPECTROMETRY", Analytical chemistry, 70(2), 1998, pp. 405-408

Abstract

Electrospray ionization Fourier transform ion cyclotron resonance (FI? CR) mass spectrometry is capable of producing high mass resolving power and improved mass accuracy for large proteins and noncovalent complexes when coupled with collisionally induced dissociation (CID) of noncovalent adducts and consequent minimization of ion charge density in the ICR trap during measurements. This work demonstrates the application of in-trap cleanup to several biologically relevant systems, including carbonic anhydrase, 4-oxalocrotonate tautomerase (4OT) analogue, and SecB, a chaperone from Escherichia coli. In-trap cleanup yields improved mass measurements for these systems and is expected to further enable measurements for even more complex systems where adduction levels have precluded study of intact complexes.

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Documento generato il 07/07/20 alle ore 10:43:48