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Titolo:
THE NEUROSPORA AAB-1 GENE ENCODES A CCAAT BINDING-PROTEIN HOMOLOGOUS TO YEAST HAP5
Autore:
CHEN HX; CRABB JW; KINSEY JA;
Indirizzi:
UNIV KANSAS,MED CTR,DEPT MICROBIOL MOL GENET & IMMUNOL,3901 RAINBOW BLVD KANSAS CITY KS 66160 W ALTON JONES CELL SCI CTR LAKE PLACID NY 12946
Titolo Testata:
Genetics
fascicolo: 1, volume: 148, anno: 1998,
pagine: 123 - 130
SICI:
0016-6731(1998)148:1<123:TNAGEA>2.0.ZU;2-5
Fonte:
ISI
Lingua:
ENG
Soggetto:
AM GDH GENE; SACCHAROMYCES-CEREVISIAE; TRANSCRIPTIONAL ACTIVATOR; HETEROLOGOUS SUBUNITS; SEQUENCE IDENTITY; DNA COMPLEX; CBF-B; CRASSA; UPSTREAM; CLONING;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
45
Recensione:
Indirizzi per estratti:
Citazione:
H.X. Chen et al., "THE NEUROSPORA AAB-1 GENE ENCODES A CCAAT BINDING-PROTEIN HOMOLOGOUS TO YEAST HAP5", Genetics, 148(1), 1998, pp. 123-130

Abstract

The expression of the am (glutamate dehydrogenase) gene is dependent upon two upstream activating sequences, designated URSam alpha and URSam beta. A heteromeric nuclear protein Am Alpha Binding protein (AAB) binds specifically to a CCAAT box within the URSam alpha element. AAB appears to be composed of three components. We used polyclonal antiserum raised against the highly purified AAB1 subunit to isolate a partial aab-1 cDNA clone, which was then used to isolate a full-length cDNA and a genomic clone. The full-length cDNA has the potential to encode a 272 amino acid protein with a calculated molecular weight of 30 kD. Amino acid sequence obtained by Edman analysis of the AAB1 protein confirmed that the aab-1 gene had been cloned. AAB-1 shows similarity to the HAP5 protein of yeast and the CBF-C protein of rat. Each of these proteins is an essential subunit of their respective heteromeric CCAATbinding proteins. The aab1 gene maps on linkage group III of Neurospora crassa near the trp-1 locus. Disruption of the aab-1 gene results in pleiotropic effects on growth and development as well as a 50% reduction in glutamate dehydrogenase levels. Transformation of the aab-1 disruption mutant strain with the cloned genomic copy of the aab-1 gene rescued all of the phenotypic alterations associated with the aab-1 mutation.

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Documento generato il 16/07/20 alle ore 20:01:55