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Titolo:
DEVELOPMENT OF REFERENCE PROCEDURES FOR BROTH MICRODILUTION ANTIFUNGAL SUSCEPTIBILITY TESTING OF YEASTS WITH STANDARDIZED END-POINT DETERMINATION
Autore:
MAKIMURA K; SUDO T; KUDO M; UCHIDA K; YAMAGUCHI H;
Indirizzi:
TEIKYO UNIV,INST MED MYCOL,359 OTSUKA TOKYO 19203 JAPAN
Titolo Testata:
Microbiology and immunology
fascicolo: 1, volume: 42, anno: 1998,
pagine: 55 - 59
SICI:
0385-5600(1998)42:1<55:DORPFB>2.0.ZU;2-Q
Fonte:
ISI
Lingua:
ENG
Soggetto:
AMPHOTERICIN-B; NATIONAL-COMMITTEE; MACRODILUTION; FLUCONAZOLE; CANDIDA; AGENTS; SYSTEM;
Keywords:
ANTIFUNGAL SUSCEPTIBILITY TEST; MIC; NCCLS; END-POINT;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
18
Recensione:
Indirizzi per estratti:
Citazione:
K. Makimura et al., "DEVELOPMENT OF REFERENCE PROCEDURES FOR BROTH MICRODILUTION ANTIFUNGAL SUSCEPTIBILITY TESTING OF YEASTS WITH STANDARDIZED END-POINT DETERMINATION", Microbiology and immunology, 42(1), 1998, pp. 55-59

Abstract

Standard guidelines for the broth microdilution antifungal susceptibility testing of amphotericin B, flucytosine, fluconazole, miconazole and itraconazole are reported. These are a modification of the method developed by the National Committee for Clinical Laboratory Standards (NCCLS) on the following two points: standardization of the means of endpoint determination and the inclusion of miconazole and itraconazole in the testing. MIC was determined to be when the positive control hada turbidity of 0.2 at the 630 nm wavelength. The endpoint was 80% inhibition for azoles and 100% inhibition for other drugs. The method provided good reproducibility, and a wide range of MIC distribution was observed in all antifungal agents except amphotericin B.

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Documento generato il 01/12/20 alle ore 10:30:42