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Titolo:
IDENTIFICATION OF URINARY DIPEPTIDASE AS THE RELEASED FORM OF RENAL DIPEPTIDASE
Autore:
WE JS; KANG BY; LEE JC; LEE HB; PARK HS;
Indirizzi:
CHONNAM NATL UNIV,COLL PHARM,DEPT PHARM,YONGBONG DONG KWANGJU 500757 SOUTH KOREA CHONNAM NATL UNIV,COLL PHARM,DEPT PHARM KWANGJU 500757 SOUTH KOREA CHONNAM NATL UNIV,COLL NAT SCI,DEPT BIOL KWANGJU SOUTH KOREA
Titolo Testata:
Kidney & blood pressure research
fascicolo: 6, volume: 20, anno: 1997,
pagine: 411 - 415
SICI:
1420-4096(1997)20:6<411:IOUDAT>2.0.ZU;2-4
Fonte:
ISI
Lingua:
ENG
Soggetto:
KIDNEY MICROVILLAR MEMBRANE; DEHYDROPEPTIDASE-I; CARBAPENEM ANTIBIOTICS; MICROSOMAL DIPEPTIDASE; AFFINITY PURIFICATION; MOLECULAR-CLONING; PHOSPHOLIPASE-C; ECTO-ENZYMES; GLUTATHIONE; EXPRESSION;
Keywords:
URINARY DIPEPTIDASE; RENAL DIPEPTIDASE; GPI PROTEIN;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
36
Recensione:
Indirizzi per estratti:
Citazione:
J.S. We et al., "IDENTIFICATION OF URINARY DIPEPTIDASE AS THE RELEASED FORM OF RENAL DIPEPTIDASE", Kidney & blood pressure research, 20(6), 1997, pp. 411-415

Abstract

Amphipathic and hydrophilic forms of human renal dipeptidase and urinary dipeptidase were purified by affinity chromatography using cilastatin, a dipeptidase inhibitor, as the ligand. The sequence analyses of the first ten amino acids of renal and urinary dipeptidases were shownto be identical, and they are Asp-Phe-Phe-Arg-Asp-Glu-Ala-Glu-Arg-Ile. Unambiguous results of amino acid sequencing, the molecular weight of native protein (190 kD), the molecular weight of subunit (47.7 kD) and a single band in sodium dodecyl sulfate-polyacrylamide gel electrophoresis indicate that the enzymes are composed of homotetramers. This is the most direct evidence that urinary dipeptidase is the released form of renal dipeptidase. In fact, they are the same enzymes.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 30/11/20 alle ore 00:43:05