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Titolo:
WORTMANNIN BLOCKS GOLDFISH RETINAL PHOSPHATIDYLINOSITOL 3-KINASE AND NEURITE OUTGROWTH
Autore:
LAVIE Y; DYBOWSKI J; AGRANOFF BW;
Indirizzi:
UNIV MICHIGAN,NEUROSCI LAB,1103 E HURON ANN ARBOR MI 48104 UNIV MICHIGAN,NEUROSCI LAB ANN ARBOR MI 48104
Titolo Testata:
Neurochemical research
fascicolo: 4, volume: 22, anno: 1997,
pagine: 373 - 378
SICI:
0364-3190(1997)22:4<373:WBGRP3>2.0.ZU;2-4
Fonte:
ISI
Lingua:
ENG
Soggetto:
OPTIC-NERVE; PHOSPHOINOSITIDE 3-KINASE; EXPLANT CULTURE; GANGLION-CELLS; REGENERATION; INHIBITOR; PROTEIN; KINASE; PURIFICATION; EXPRESSION;
Keywords:
GOLDFISH RETINA; NEURITE OUTGROWTH; NEURONAL REGENERATION; PHOSPHATIDYLINOSITOL 3-KINASE; WORTMANNIN;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
39
Recensione:
Indirizzi per estratti:
Citazione:
Y. Lavie et al., "WORTMANNIN BLOCKS GOLDFISH RETINAL PHOSPHATIDYLINOSITOL 3-KINASE AND NEURITE OUTGROWTH", Neurochemical research, 22(4), 1997, pp. 373-378

Abstract

The goldfish retina has been used extensively for the study of nerve regeneration. A role for phosphatidylinositol 3-kinase (PI3K) in neurite outgrowth from goldfish retinal explants has been examined by meansof wortmannin (WT), a selective inhibitor of the enzyme. The presenceof PI3K in retinal extracts was determined by means of immunoprecipitation as well as by an in vitro assay system for catalytic activity. The relative amount of the p85 subunit of PI3K detected by western blotin the retina following optic nerve crush was unchanged. WT inhibitedgoldfish brain PI3K activity at concentrations as low as 10(-9) M, approximating that reported for inhibition of mammalian PI3K's. Daily addition of 10(-8) M WT to retinal explants, activated by prior crush ofthe optic nerve, significantly inhibited neurite outgrowth during a 7day in vitro culture period, while a single addition of WT to freshlyexplanted retina had no effect on neurite outgrowth. These results suggest that a PI3K-mediated process may be critical for nerve regrowth.

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Documento generato il 26/09/20 alle ore 08:17:51