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Titolo:
EFFECTS OF DIFFERENT HYALURONIDASE CONCENTRATIONS AND MECHANICAL PROCEDURES FOR CUMULUS CELL REMOVAL ON THE OUTCOME OF INTRACYTOPLASMIC SPERM INJECTION
Autore:
VANDEVELDE H; NAGY ZP; JORIS H; DEVOS A; VANSTEIRTEGHEM AC;
Indirizzi:
FREE UNIV BRUSSELS,CTR REPROD MED,LAARBEEKLAAN 101 B-1090 BRUSSELS BELGIUM
Titolo Testata:
Human reproduction
fascicolo: 10, volume: 12, anno: 1997,
pagine: 2246 - 2250
SICI:
0268-1161(1997)12:10<2246:EODHCA>2.0.ZU;2-I
Fonte:
ISI
Lingua:
ENG
Soggetto:
HUMAN OOCYTES; ASSISTED FERTILIZATION; INVITRO FERTILIZATION; PARTHENOGENETIC ACTIVATION; SUBZONAL INSEMINATION; EARLY-PREGNANCY; LUTEAL PHASE; FEMALE AGE; RATES; EMBRYOS;
Keywords:
CUMULUS CELLS; HYALURONIDASE; ICSI; OOCYTE; PIPETTE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
33
Recensione:
Indirizzi per estratti:
Citazione:
H. Vandevelde et al., "EFFECTS OF DIFFERENT HYALURONIDASE CONCENTRATIONS AND MECHANICAL PROCEDURES FOR CUMULUS CELL REMOVAL ON THE OUTCOME OF INTRACYTOPLASMIC SPERM INJECTION", Human reproduction, 12(10), 1997, pp. 2246-2250

Abstract

The aim of this study was Po compare concentrations of hyaluronidase and mechanical methods used to denude human oocytes from surrounding cumulus and corona cells prior to intracytoplasmic sperm injection (ICSI). Cumulus and corona cells were removed in two pipetting steps: first in a medium containing hyaluronidase, and then in a medium without enzyme, The first step in the procedure was investigated, Different hyaluronidase concentrations (rs, 39 or 10 IU/ml) and pipettes of different size (inner diameter 250 or >1000 mu m) were used to remove the cumulus cells, The time required to denude the oocytes was recorded. Metaphase II oocytes were injected, and the survival, fertilization, embryo development and pregnancy rates were evaluated. We found that by using a pipette with an inner diameter of at least 1000 mu m we mere ableto decrease significantly the time an oocyte is exposed to hyaluronidase, even if the concentration of enzyme is very low (10 IU/ml). For the different conditions there was no statistically significant effect on the outcome in terms of survival, normal fertilization [two pronuclear (2PN)], parthenogenetic activation (1PN), abnormal fertilization (3PN), embryo development and pregnancy rates after ICSI. In conclusion, a concentration as low as 10 IU/ml hyaluronidase in combination witha pipette of at least 1000 FM inner diameter can be used successfullyto denude oocytes fur microinjection.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 30/11/20 alle ore 03:15:02