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Titolo:
THE 2 GUANINE-NUCLEOTIDE EXCHANGE FACTOR DOMAINS OF TRIO LINK THE RAC1 AND THE RHOA PATHWAYS IN-VIVO
Autore:
BELLANGER JM; LAZARO JB; DIRIONG S; FERNANDEZ A; LAMB N; DEBANT A;
Indirizzi:
CNRS,CRBM,ERS155,1919 ROUTE MENDE,BP5051 F-34033 MONTPELLIER 01 FRANCE CNRS,CRBM,ERS155 F-34033 MONTPELLIER 01 FRANCE
Titolo Testata:
Oncogene
fascicolo: 2, volume: 16, anno: 1998,
pagine: 147 - 152
SICI:
0950-9232(1998)16:2<147:T2GEFD>2.0.ZU;2-D
Fonte:
ISI
Lingua:
ENG
Soggetto:
ACTIN STRESS FIBERS; BINDING PROTEIN-RHO; CDC42 GTPASES; ACTIVATION; KINASE; RAS; ONCOGENE; TRANSFORMATION; FAMILY;
Keywords:
TRIO; GDP/GTP EXCHANGE FACTOR; ACTIN; MAP KINASES;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
32
Recensione:
Indirizzi per estratti:
Citazione:
J.M. Bellanger et al., "THE 2 GUANINE-NUCLEOTIDE EXCHANGE FACTOR DOMAINS OF TRIO LINK THE RAC1 AND THE RHOA PATHWAYS IN-VIVO", Oncogene, 16(2), 1998, pp. 147-152

Abstract

Trio contains two functional guanine nucleotide exchange factors (GEF) domains for the Rho-like GTPases and a serine/threonine kinase domain. In vitro, GEF domain 1(GEFD1) is specifically active on Rac1, whileGEF domain 2 (GEFD2) targets RhoA. To determine whether Trio could activate Rad and RhoA in vivo, we measured the effect of Trio on MitogenActivated Protein Kinase (MAPK) pathways and cytoskeletal rearrangments events mediated by the two GTPases. We show that: (i) the GEFD1 domain of Trio triggers the MAPK pathway leading to Jun kinase (JNK) activation and the production of membrane ruffles; (ii) co-expression of the TrioGEFD1 domain with a dominant-negative form of Rac blocked JNK induction, whereas a dominant-negative form of Cdc42 did not; (iii) a deletion mutant of TrioGEFD1 lacking a region important for exchange activity could not stimulate JNK activity; (iv) in contrast, the TrioGEFD2 domain does not stimulate JNK activity and induces the formation ofstress fibers, as does activated RhoA; (v) furthermore, co-expressionof both GEF domains induces simultaneously the formation of ruffles and stress fibers. Trio, therefore represents a unique member of the Rho-GEFs family possessing two functional domains of distinct specificities, that allow it to link Rho and Rac signaling pathway in vivo.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 27/11/20 alle ore 10:20:20