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Titolo:
IN-VITRO ANALYSIS OF GADOLINIUM CHLORIDE ABROGATION OF THE SYSTEMIC TOLERANCE INDUCED BY PORTAL VENOUS ADMINISTRATION OF ULTRAVIOLET B-IRRADIATED DONOR CELLS
Autore:
YU S; NAKAFUSA Y; FLYE MW;
Indirizzi:
WASHINGTON UNIV,SCH MED,DEPT SURG,1 BARNES HOSP PLAZA,SUITE 5103 ST LOUIS MO 63110 WASHINGTON UNIV,SCH MED,DEPT SURG ST LOUIS MO 63110
Titolo Testata:
Transplantation
fascicolo: 12, volume: 64, anno: 1997,
pagine: 1684 - 1688
SICI:
0041-1337(1997)64:12<1684:IAOGCA>2.0.ZU;2-7
Fonte:
ISI
Lingua:
ENG
Soggetto:
CARDIAC ALLOGRAFT SURVIVAL; ALLOGENEIC CELLS; HYPERSENSITIVITY RESPONSES; KUPFFER CELLS; INDUCTION; RAT; INOCULATION; BLOCKADE; SUPPRESSION; TRANSPLANTATION;
Tipo documento:
Article
Natura:
Periodico
Citazioni:
27
Recensione:
Indirizzi per estratti:
Citazione:
S. Yu et al., "IN-VITRO ANALYSIS OF GADOLINIUM CHLORIDE ABROGATION OF THE SYSTEMIC TOLERANCE INDUCED BY PORTAL VENOUS ADMINISTRATION OF ULTRAVIOLET B-IRRADIATED DONOR CELLS", Transplantation, 64(12), 1997, pp. 1684-1688

Abstract

Background Normally, a Buffalo (BUF) recipient (RT1(b)) rejects a heterotopically transplanted Lewis (LEW) heart (RT1(1)) drained into the portal vein (PV) within 14 days, However, the addition of PV administration of 25x10(6) ultraviolet B (UVB)-treated LEW spleen cells (SC) toBUF recipients 7 days before cardiac transplantation results in 70% long-term allograft survival. Methods. In this study, we used gadolinium chloride (GdCl3) (7 mg/kg/day) to selectively block the phagocytosisof recipient hepatic Kupffer cells before PV injection of UVB-treateddonor SC to examine the mechanism of tolerance induction, as measuredby in vitro analysis of mixed lymphocyte culture (MLC), T cell cytotoxicity, T helper cell precursors (pTH), and cytotoxic T cell precursors (pCTL) by limiting dilution analysis, Results. A BUF recipient that received untreated or gamma-irradiated LEW SC intraportally reacted toin vitro stimulation by LEW alloantigen with increased MLC proliferation, T cell cytotoxicity, pTH and pCTL frequencies, and interleukin-2 production. In contrast, SC from BUF that received UVB-treated LEW SC were hyporesponsive on MLC stimulation by donor LEW alloantigen and exhibited markedly reduced cytotoxicity, pTH and pCTL frequency, and interleukin-a production. However, normal in vitro responsiveness resulted with stimulation by third-party Brown-Norway (RT1(n)) SC, thus indicating that the systemic hyporesponsiveness was specific for the UVB donor alloantigen given PV, On the other hand, GdCl3, given by intravenous injection daily for 3 days before PV alloantigen blocked the induction of in vitro hyporesponsiveness. Conclusion. Therefore, prevention of alloantigen sequestration by GdCl3, inhibition of hepatic Kupffer cell phagocytosis was pivotal in preventing the development of portal venous tolerance.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 11/07/20 alle ore 16:16:15