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Titolo:
DELAYING THE ONSET OF M-PHASE IN NIH 3T3 CELLS BLOCKED IN EARLY S-PHASE OCCURS VIA ACCUMULATING CYCLIN B1 AND TYROSINE-PHOSPHORYLATED P34(CDC2) IN THE NUCLEUS
Autore:
DAVIDPFEUTY T; NOUVIANDOOGHE Y; ROUILLARD D;
Indirizzi:
CTR UNIV ORSAY,UMR 146 CNRS,INST CURIE RECH,BATIMENT 110 F-91405 ORSAY FRANCE INST CURIE F-75231 PARIS FRANCE
Titolo Testata:
Biology of the cell
fascicolo: 3, volume: 89, anno: 1997,
pagine: 179 - 197
SICI:
0248-4900(1997)89:3<179:DTOOMI>2.0.ZU;2-K
Fonte:
ISI
Lingua:
ENG
Soggetto:
CDK-ACTIVATING KINASE; CDC2 PROTEIN-KINASE; DNA-REPLICATION; SUBCELLULAR-LOCALIZATION; CHROMOSOME CONDENSATION; CATALYTIC SUBUNIT; DEPENDENT KINASES; P34CDC2 KINASE; HUMAN HOMOLOG; HUMAN WEE1;
Keywords:
CELL CYCLE REGULATION; S-TO-M COUPLING; CELL CYCLE PROTEIN LOCALIZATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
65
Recensione:
Indirizzi per estratti:
Citazione:
T. Davidpfeuty et al., "DELAYING THE ONSET OF M-PHASE IN NIH 3T3 CELLS BLOCKED IN EARLY S-PHASE OCCURS VIA ACCUMULATING CYCLIN B1 AND TYROSINE-PHOSPHORYLATED P34(CDC2) IN THE NUCLEUS", Biology of the cell, 89(3), 1997, pp. 179-197

Abstract

An affinity-purified antibody (anti-Cdc2C) raised against the carboxyterminal sequence LDNQIKKM of p34(cdc2) uncovered in NIH 3T3 cells a protein subpopulation, the location and the level of accumulation of which evolve during progression through the cell cycle: it first emerges inside the nucleus in late G1/early S phase and continues to build up principally in this location throughout S phase; a cytoplasmic expression then becomes apparent near the end of S phase, develops during G2 and sometimes prevails over the nuclear expression; it finally relocates to the nucleus in early prophase. We propose that a major part ofthis subpopulation would represent p34(cdc2) molecules existing inside a complex with cyclin B1. NIH 3T3 cells arrested in early S phase with aphidicolin do not commit prematurely to mitosis which indicates that the regulatory pathway involved in preserving the temporal order ofS and M phases is functioning in these conditions. Conjugated Westernblot analysis and immunofluorescence microscopy showed that cyclin A,cyclin B1 and tyrosine-phosphorylated p34(cdc2) continue to build up predominantly in the nucleus of the arrested cells. After release fromthe block, the cells rapidly reenter S and G2 phases and, concomitantly, cyclin B1 and tyrosine-phosphorylated p34(cdc2) relocate to the cytoplasm before redistributing again in the nucleus in early prophase. These data would suggest that delaying the onset of M phase in NIH 3T3cells in which the rate of DNA replication is reduced, is first ensured by a mechanism that prevents the cytoplasmic relocation of inactivep34(cdc2)/cyclin B1 complexes continually forming in the nucleus oncethe G1 period of mitotic cyclin instability is over.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 13/08/20 alle ore 00:04:37