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Titolo:
THE SOLUTION STRUCTURE OF FUNCTIONALLY ACTIVE HUMAN PROLIFERATING CELL NUCLEAR ANTIGEN DETERMINED BY SMALL-ANGLE NEUTRON-SCATTERING
Autore:
SCHURTENBERGER P; EGELHAAF SU; HINDGES R; MAGA G; JONSSON ZO; MAY RP; GLATTER O; HUBSCHER U;
Indirizzi:
ETH ZURICH,INST POLYMERE CH-8092 ZURICH SWITZERLAND INST MAX VON LAUE PAUL LANGEVIN F-38042 GRENOBLE 9 FRANCE UNIV ZURICH,INST VET BIOCHEM CH-8057 ZURICH SWITZERLAND KARL FRANZENS UNIV GRAZ,INST PHYS CHEM A-8010 GRAZ AUSTRIA
Titolo Testata:
Journal of Molecular Biology
fascicolo: 1, volume: 275, anno: 1998,
pagine: 123 - 132
SICI:
0022-2836(1998)275:1<123:TSSOFA>2.0.ZU;2-1
Fonte:
ISI
Lingua:
ENG
Soggetto:
DNA-POLYMERASE-DELTA; REPLICATION FACTOR-C; ESCHERICHIA-COLI; SLIDING CLAMPS; CALF THYMUS; FACTOR PCNA; PROTEIN; INHIBITOR; EPSILON; REPAIR;
Keywords:
PROLIFERATING CELL NUCLEAR ANTIGEN; SMALL-ANGLE NEUTRON SCATTERING; SOLUTION STRUCTURE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
40
Recensione:
Indirizzi per estratti:
Citazione:
P. Schurtenberger et al., "THE SOLUTION STRUCTURE OF FUNCTIONALLY ACTIVE HUMAN PROLIFERATING CELL NUCLEAR ANTIGEN DETERMINED BY SMALL-ANGLE NEUTRON-SCATTERING", Journal of Molecular Biology, 275(1), 1998, pp. 123-132

Abstract

The function of proliferating cell nuclear antigen (PCNA) in DNA replication and repair is to form a sliding clamp with replication factor C (RF-C) tethering DNA polymerase delta or epsilon to DNA. In addition, PCNA has been found to interact directly with various proteins involved in cell cycle regulation. The crystal structure of yeast PCNA shows that the protein forms a homotrimeric ring lining a hole through which double-stranded DNA can thread, thus forming a moving platform for DNA synthesis. Human and yeast PCNA are highly conserved at a structural and functional level. We determined the solution structure of functionally active human PCNA by small-angle neutron scattering. Our measurements strongly support a trimeric ring-like structure of functionally active PCNA in solution, and the data are in good agreement with model calculations based on the crystal structure from yeast PCNA. The human PCNA used in the small-angle neutron scattering experiments was active before and after the measurements in a RF-C independent and a RF-C dependent assay suggesting that the trimeric structure is the in vivo functional form. (C) 1998 Academic Press Limited.

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Documento generato il 26/09/20 alle ore 18:27:37