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Titolo:
THE KOSMOTROPIC (STRUCTURE-FORMING) EFFECT OF COMPENSATORY SOLUTES
Autore:
GALINSKI EA; STEIN M; AMENDT B; KINDER M;
Indirizzi:
UNIV BONN,INST MIKROBIOL & BIOTECHNOL,MECKENHEIMER ALLEE 168 D-53115 BONN GERMANY UNIV BONN,INST THEORET BIOL D-53115 BONN GERMANY
Titolo Testata:
Comparative biochemistry and physiology. Section A: Comparative physiology
fascicolo: 3, volume: 117, anno: 1997,
pagine: 357 - 365
SICI:
1095-6433(1997)117:3<357:TK(EOC>2.0.ZU;2-N
Fonte:
ISI
Lingua:
ENG
Soggetto:
PREFERENTIAL HYDRATION; HALOPHILIC EUBACTERIA; WATER-STRESS; OSMOLYTES; PROTEINS; STABILIZATION; SOLVENTS; BEHAVIOR;
Keywords:
HALOPHILIC BACTERIA; COMPATIBLE/COMPENSATORY SOLUTE; ORGANIC OSMOLYTE; OSMOADAPTATION; WATER-SOLUTE INTERACTION; HYDRATION NUMBER; NEAR-INFRARED SPECTROSCOPY;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
31
Recensione:
Indirizzi per estratti:
Citazione:
E.A. Galinski et al., "THE KOSMOTROPIC (STRUCTURE-FORMING) EFFECT OF COMPENSATORY SOLUTES", Comparative biochemistry and physiology. Section A: Comparative physiology, 117(3), 1997, pp. 357-365

Abstract

The production and/or accumulation of organic osmolytes, which serve to compensate for osmotic pressure and low cytoplasmic water activity,are the typical properties of many halophilic microorganisms. These so-called compatible/compensatory solutes not only maintain osmotic equilibrium but also protect and stabilize cytoplasmic components againsta variety of stress factors. A molecular basis for this is seen in the kosmotropic nature of these solutes, referring to the structure-forming ability in water. Using a gel filtration method and near-infrared spectroscopy, we were able to demonstrate that nature's prime compensatory solutes (betaine, ectoines,-proline, N-acetylated diamino acids) strongly influence surrounding water molecules. The hydration numbers observed (three to five molecules of water per molecule of solute) arecomparable with those of the ''unfreezable water'' recently reported for trehalose and are markedly higher than those of disturbing (chaotropic) salts. In addition, a Gaussian analysis of hydration spectra revealed vibration bands similar to those observed in frozen water, indicating that strong hydrogen bonds are induced by the presence of compensatory solutes. (C) 1997 Elsevier Science Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 27/10/20 alle ore 02:09:37