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Titolo:
TEMPERATURE-DEPENDENT NONSPECIFIC ADSORPTION OF RECOMBINANT BOVINE LEUKEMIA-VIRUS RECEPTOR BLVRCP1 IN IMMUNOASSAY
Autore:
ORLIK O; ALTANER C; SPLITTER GA;
Indirizzi:
UNIV WISCONSIN,DEPT ANIM HLTH & BIOMED SCI,1655 LINDEN DR MADISON WI 53706 UNIV WISCONSIN,DEPT ANIM HLTH & BIOMED SCI MADISON WI 53706 SLOVAK ACAD SCI,CANC RES INST,DEPT MOL VIROL BRATISLAVA 81232 SLOVAKIA
Titolo Testata:
Journal of immunoassay
fascicolo: 2, volume: 18, anno: 1997,
pagine: 185 - 198
SICI:
0197-1522(1997)18:2<185:TNAORB>2.0.ZU;2-8
Fonte:
ISI
Lingua:
ENG
Soggetto:
LINKED IMMUNOSORBENT-ASSAY; IMMUNOGLOBULIN-G; CELL-RECEPTOR; POLYSTYRENE; ANTIBODIES; BINDING; ELISA; GENE;
Keywords:
IMMUNOASSAY; ANTIGEN CAPTURE ASSAY; ELISA; RIA; IRMA; MONOCLONAL ANTIBODIES; BOVINE LEUKEMIA VIRUS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
15
Recensione:
Indirizzi per estratti:
Citazione:
O. Orlik et al., "TEMPERATURE-DEPENDENT NONSPECIFIC ADSORPTION OF RECOMBINANT BOVINE LEUKEMIA-VIRUS RECEPTOR BLVRCP1 IN IMMUNOASSAY", Journal of immunoassay, 18(2), 1997, pp. 185-198

Abstract

Recombinant bovine leukemia virus receptor, BLVRcp1, possessed the unusual property of binding plastic plates after blocking nonspecific binding sites. Adhesiveness of BLVRcp1 to blocked plates hindered development of an antigen capture and receptor binding assay with this protein. Unexpectedly, non-specific adsorption of BLVRcp1 was dramatically influenced by temperature. Optimizing incubation temperature and antigen capture at 4 degrees C instead of 37 degrees C and the use of milk as blocking solution removed nonspecific binding of BLVRcp1 allowing development of a functional immunoassay. Thus, the temperature used forantigen capture can be a critical factor that influences performance of the immunoassay.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 27/11/20 alle ore 04:22:04