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Titolo:
COPROANTIGEN DETECTION IN DOGS EXPERIMENTALLY AND NATURALLY INFECTED WITH ECHINOCOCCUS-GRANULOSUS BY A MONOCLONAL ANTIBODY-BASED ENZYME-LINKED-IMMUNOSORBENT-ASSAY
Autore:
MALGOR R; NONAKA N; BASMADJIAN I; SAKAI H; CARAMBULA B; OKU Y; CARMONA C; KAMIYA M;
Indirizzi:
UNIV REPUBLICA,INST HIGIENE,FAC CIENCIAS,UNIDAD BIOL PARASITARIA,AVDAALFREDO NAVARRO 3051 MONTEVIDEO URUGUAY HOKKAIDO UNIV,GRAD SCH VET MED,PARASITOL LAB SAPPORO HOKKAIDO 060 JAPAN
Titolo Testata:
International journal for parasitology
fascicolo: 12, volume: 27, anno: 1997,
pagine: 1605 - 1612
SICI:
0020-7519(1997)27:12<1605:CDIDEA>2.0.ZU;2-1
Fonte:
ISI
Lingua:
ENG
Keywords:
ECHINOCOCCUS GRANULOSUS; COPROANTIGEN; DIAGNOSIS; DOGS; ELISA;
Tipo documento:
Article
Natura:
Periodico
Citazioni:
10
Recensione:
Indirizzi per estratti:
Citazione:
R. Malgor et al., "COPROANTIGEN DETECTION IN DOGS EXPERIMENTALLY AND NATURALLY INFECTED WITH ECHINOCOCCUS-GRANULOSUS BY A MONOCLONAL ANTIBODY-BASED ENZYME-LINKED-IMMUNOSORBENT-ASSAY", International journal for parasitology, 27(12), 1997, pp. 1605-1612

Abstract

A sandwich ELISA for the detection of Echinococcus granulosus coproantigen in formalin and heat-treated faecal supernatants of dogs was developed. The assay used affinity-purified polyclonal antibodies obtained from rabbits hyperimmunised with E. granulosus excretory/secretory antigens and biotinylated monoclonal antibody EmA9 produced against adult E. multilocularis somatic extract. The test was sensitive to 7 ng and 2.3 ng of E. granulosus protein and carbohydrate/ml of faecal supernatant, respectively. Thirteen helminth-free dogs were infected with different amounts of E. granulosus protoscoleces and the presence of coproantigen was monitored during the prepatent period until day 35 post-infection, when they were necropsied. Faecal antigen levels started to rise above the normal range between days 10 and 20 post-infection, and typically peaked at the end of the experiment. All the dogs, bearing from 3 to 67 700 worms, showed positive values in the ELISA during the prepatent period. One dog experimentally infected with Taenia hydatigena metacestode and harbouring three worms, tested positive only after the prepatent period at day 52. The test was applied to 98 stray dogs. The ELISA detected all of four dogs naturally infected with E. granulosus, two dogs with patent infections of T. hydatigena and two dogswith no cestode infections, showing a sensitivity of 100% and a specificity of 96%. (C) 1997 Australian Society for Parasitology. Publishedby Elsevier Science Ltd.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 20/01/20 alle ore 08:16:56