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Titolo:
POLYSIALYLATED NEURAL CELL-ADHESION IS INVOLVED IN TARGET-INDUCED MORPHOLOGICAL-DIFFERENTIATION OF ARCUATE DOPAMINERGIC-NEURONS
Autore:
LOUDES C; ROUGON G; KORDON C; FAIVREBAUMAN A;
Indirizzi:
INSERM,U159,2 TER RUE ALESIA F-75014 PARIS FRANCE CNRS,UPR 9443 F-13288 MARSEILLE 09 FRANCE
Titolo Testata:
European journal of neuroscience
fascicolo: 11, volume: 9, anno: 1997,
pagine: 2323 - 2333
SICI:
0953-816X(1997)9:11<2323:PNCIII>2.0.ZU;2-K
Fonte:
ISI
Lingua:
ENG
Soggetto:
HYDROXYLASE-IMMUNOREACTIVE NEURONS; MESSENGER-RIBONUCLEIC-ACID; SUBSTANTIA-NIGRA; MEDIAN-EMINENCE; GROWTH-HORMONE; NERVOUS-SYSTEM; ADULT-RATS; MOLECULE; HYPOPHYSEAL; EXPRESSION;
Keywords:
PERIVENTRICULAR NUCLEUS; RAT; PARS INTERMEDIA; TYROSINE HYDROXYLASE; MORPHOMETRY;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
43
Recensione:
Indirizzi per estratti:
Citazione:
C. Loudes et al., "POLYSIALYLATED NEURAL CELL-ADHESION IS INVOLVED IN TARGET-INDUCED MORPHOLOGICAL-DIFFERENTIATION OF ARCUATE DOPAMINERGIC-NEURONS", European journal of neuroscience, 9(11), 1997, pp. 2323-2333

Abstract

We have previously shown that the morphological and biochemical maturation of developing rat hypothalamic dopaminergic neurons is accelerated when they are cocultivated with pituitary intermediate robe cells, one of their targets. Only two subsets of hypothalamic dopaminergic neurons (arcuate, A12, and periventricular, A14, nuclei) may project to the pars intermedia. In order to determine whether the two populationsare equally responsive to coculture conditions, we microdissected thehypothalamus of 17-day-old rat fetuses in two fragments containing cell bodies from the A12 and from the A14 regions, prepared neuronal cultures from both portions and incubated them separately with intermediate robe cells. The presence of intermediate lobe cells increased tyrosine hydroxylase levels in both dopaminergic neuron subsets, but morphological differentiation was accelerated in dopaminergic neurons originating in the arcuate nucleus only. We then investigated whether physical contact between developing arcuate neurons and their target cells was a prerequisite of the morphological effect by interposing a semipermeable membrane between cultivated neurons and intermediate lobe cellsin transwell culture dishes. The morphological effect was no longer observed under transwell coculture conditions, pointing to the involvement of membrane-bound molecules. Accordingly, the stimulating effect of coculture on arcuate dopaminergic neurons was completely abolished by the removal of polysialic acid on neural cell adhesion molecules by endoneuraminidase N treatment. Thus, maturation of A12 and A14 dopaminergic neurons exhibits differential susceptibility to intermediate robe target cells, and polysialylated-NCAM is required for the contact-dependent effect.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 09/04/20 alle ore 10:38:53