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Titolo:
FGF-2 IS SUFFICIENT TO ISOLATE PROGENITORS FOUND IN THE ADULT MAMMALIAN SPINAL-CORD
Autore:
SHIHABUDDIN LS; RAY J; GAGE FH;
Indirizzi:
SALK INST BIOL STUDIES,GENET LAB,10010 N TORREY PINES RD LA JOLLA CA 92037
Titolo Testata:
Experimental neurology
fascicolo: 2, volume: 148, anno: 1997,
pagine: 577 - 586
SICI:
0014-4886(1997)148:2<577:FISTIP>2.0.ZU;2-Y
Fonte:
ISI
Lingua:
ENG
Soggetto:
FIBROBLAST GROWTH-FACTOR; NEURONAL PRECURSOR CELLS; CENTRAL-NERVOUS-SYSTEM; DENTATE GYRUS; MOUSE BRAIN; STEM-CELLS; SUBVENTRICULAR ZONE; CELLULAR DIVERSITY; SUBEPENDYMAL LAYER; FACTOR RECEPTOR;
Keywords:
FGF-2; SPINAL CORD; ADULT PROGENITORS; NEURONS; ASTROCYTES; OLIGODENDROCYTES;
Tipo documento:
Article
Natura:
Periodico
Citazioni:
44
Recensione:
Indirizzi per estratti:
Citazione:
L.S. Shihabuddin et al., "FGF-2 IS SUFFICIENT TO ISOLATE PROGENITORS FOUND IN THE ADULT MAMMALIAN SPINAL-CORD", Experimental neurology, 148(2), 1997, pp. 577-586

Abstract

The adult rat brain contains progenitor cells that can be induced to proliferate in vitro in response to FGF-2. In the present study we explored whether similar progenitor cells can be cultured from different levels (cervical, thoracic, lumbar, and sacral) of adult rat spinal cord and whether they give rise to neurons and glia as well as spinal cord-specific neurons (e.g., motoneurons). Cervical, thoracic, lumbar, and sacral areas of adult rat spinal cord (>3 months old) were microdissected and neural progenitors were isolated and cultured in serum-freemedium containing FGF-2 (20 ng/ml) through multiple passages. Although all areas generated rapidly proliferating cells, the cultures were heterogeneous in nature and cell morphology varied within a given area as well as between areas. A percentage of cells from all areas of the spinal cord differentiate into cells displaying antigenic properties of neuronal, astroglial, and oligodendroglial lineages; however, the majority of cells from all regions expressed the immature proliferating progenitor marker vimentin. In established multipassage cultures, a few large, neuron-like cells expressed immunoreactivity for p75NGFr and did not express GFAP. These cells may be motoneurons. These results demonstrate that FGF-2 is mitogenic for progenitor cells from adult rat spinal cord that have the potential to give rise to glia and neurons including motoneurons. (C) 1997 Academic Press.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 21/09/20 alle ore 17:41:32