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Titolo:
QUANTIFICATION OF HIV-1 PROVIRAL DNA BY A STANDARDIZED COLORIMETRIC PCR-BASED ASSAY
Autore:
IZOPET J; TAMALET C; PASQUIER C; SANDRES K; MARCHOU B; MASSIP P; PUEL J;
Indirizzi:
CHU TOULOUSE,HOP PURPAN,VIROL LAB F-31059 TOULOUSE FRANCE HOP ENFANTS LA TIMONE,VIROL LAB F-13385 MARSEILLE FRANCE HOP PURPAN,SERV MALAD INFECT TOULOUSE FRANCE
Titolo Testata:
Journal of medical virology
fascicolo: 1, volume: 54, anno: 1998,
pagine: 54 - 59
SICI:
0146-6615(1998)54:1<54:QOHPDB>2.0.ZU;2-F
Fonte:
ISI
Lingua:
ENG
Soggetto:
HUMAN-IMMUNODEFICIENCY-VIRUS; POLYMERASE CHAIN-REACTION; BLOOD MONONUCLEAR-CELLS; TYPE-1 INFECTION; QUANTITATIVE-ANALYSIS; VIRAL BURDEN; RNA; INDIVIDUALS; VIREMIA; PLASMA;
Keywords:
HIV-1 PROVIRAL DNA; QUANTIFICATION; POLYMERASE CHAIN REACTION; LATENT HIV-1 INFECTION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
26
Recensione:
Indirizzi per estratti:
Citazione:
J. Izopet et al., "QUANTIFICATION OF HIV-1 PROVIRAL DNA BY A STANDARDIZED COLORIMETRIC PCR-BASED ASSAY", Journal of medical virology, 54(1), 1998, pp. 54-59

Abstract

A simple method was developed for measuring human immunodeficiency virus type 1 (HIV-1) proviral DNA in mononuclear cells based on the commercially available Amplicor(TM) HIV-1 polymerase chain reaction (PCR) assay and the limiting dilution method. The lowest limit of detection was four proviral genomes per 10(6) cells. The accuracy was demonstrated by using serial dilutions of LAV-8E5 cells, and the interassay variability was 0.2 log. The technique was used to measure HIV-1 proviral DNA in the peripheral blood mononuclear cells (PBMC) of 18 antiretroviral drug-naive HIV-1-positive individuals before and 4 weeks after initiating double nucleoside therapy. The DNA proviral titers at baseline(median = 3.45, range = 2.11-4.7 log copies/10(6) cells) were 2.08 log greater than the infectivity titers, but there was a correlation between these two parameters (r = 0.63, P = 0.009). The mean decrease in the proviral DNA titer after 4 weeks of therapy was 0.31 log, whereas the decrease in the infectivity titer was 0.81 log and the decrease inthe plasma RNA concentration was 1.29 log. The technique was also used to measure HIV-1 proviral DNA in the PBMC of 11 patients who had undetectable plasma HIV-1 RNA after being placed on combination antiretroviral therapy. Although proviral DNA remained detectable in all patients after 36 weeks of treatment, a gradual decline with an estimated half-life of 21-58 weeks was observed. The reliability of this simple and convenient colorimetric PCR-based technique indicates its suitability for assessing the effect of current antiretroviral regimens on the latent reservoirs of provirus. (C) 1998 Wiley-Liss, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 02/12/20 alle ore 05:18:23