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Titolo:
MUSCIMOL INCREASES ACETYLCHOLINE-RELEASE BY DIRECTLY STIMULATING ADULT STRIATAL CHOLINERGIC INTERNEURONS
Autore:
LOGIN IS; PAL SN; ADAMS DT; GOLD PE;
Indirizzi:
UNIV VIRGINIA,HLTH SCI CTR,DEPT NEUROL,LAB NEUROTRANSMITTER RES,BOX 394 CHARLOTTESVILLE VA 22908 UNIV VIRGINIA,DEPT PSYCHOL CHARLOTTESVILLE VA 22908
Titolo Testata:
Brain research
fascicolo: 1-2, volume: 779, anno: 1998,
pagine: 33 - 40
SICI:
0006-8993(1998)779:1-2<33:MIABDS>2.0.ZU;2-Z
Fonte:
ISI
Lingua:
ENG
Soggetto:
RECEPTOR-MEDIATED INHIBITION; PITUITARY PARS-INTERMEDIA; IN-VIVO MICRODIALYSIS; SYNAPTIC POTENTIALS; PYRAMIDAL CELLS; GABA; DOPAMINE; NEURONS; RESPONSES; SLICES;
Keywords:
ACETYLCHOLINE; CALCIUM; DISSOCIATED NEURONS; DOPAMINE; GABA-A; MUSCIMOL; STRIATUM; VESAMICOL;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
46
Recensione:
Indirizzi per estratti:
Citazione:
I.S. Login et al., "MUSCIMOL INCREASES ACETYLCHOLINE-RELEASE BY DIRECTLY STIMULATING ADULT STRIATAL CHOLINERGIC INTERNEURONS", Brain research, 779(1-2), 1998, pp. 33-40

Abstract

Because GabaA ligands increase acetylcholine (ACh) release from adultstriatal slices, we hypothesized that activation of GabaA receptors on striatal cholinergic interneurons directly stimulates ACh secretion. Fractional [H-3]ACh release was recorded during perifusion of acutelydissociated, [H-3]choline-labeled, adult male rat striata. The GabaA agonist, muscimol, immediately stimulated release maximally similar to300% with EC50 = similar to 1 mu M. This action was enhanced by the allosteric GabaA receptor modulators, diazepam and secobarbital, and inhibited by the GabaA antagonist, bicuculline, by ligands for D2 or muscarinic cholinergic receptors or by low calcium buffer, tetrodotoxin or vesamicol. Membrane depolarization inversely regulated muscimol-stimulated secretion. Release of endogenous and newly synthesized ACh was stimulated in parallel by muscimol without changing choline release. Muscimol pretreatment inhibited release evoked by K+ depolarization or by receptor-mediated stimulation with glutamate. Thus, GabaA receptorson adult striatal cholinergic interneurons directly stimulate voltage-and calcium-dependent exocytosis of ACh stored in vesamicol-sensitivesynaptic vesicles. The action depends on the state of membrane polarization and apparently depolarizes the membrane in turn. This functional assay demonstrates that excitatory GabaA actions are not limited to neonatal tissues. GabaA-stimulated ACh release may be prevented in situ by normal tonic dopaminergic and muscarinic input to cholinergic neurons. (C) 1998 Elsevier Science B.V.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 02/07/20 alle ore 19:02:34