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Titolo:
P140SRA-1 (SPECIFICALLY RAC1-ASSOCIATED PROTEIN) IS A NOVEL SPECIFIC TARGET FOR RAC1 SMALL GTPASE
Autore:
KOBAYASHI K; KURODA S; FUKATA M; NAKAMURA T; NAGASE T; NOMURA N; MATSUURA Y; YOSHIDAKUBOMURA N; IWAMATSU A; KAIBUCHI K;
Indirizzi:
NARA INST SCI & TECHNOL,DIV SIGNAL TRANSDUCT IKOMA 63001 JAPAN NARA INST SCI & TECHNOL,DIV SIGNAL TRANSDUCT IKOMA 63001 JAPAN JAPAN SCI & TECHNOL,PRECURSORY RES EMBRYON SCI & TECHNOL,INHERITANCE & VARIAT GRP KYOTO 61902 JAPAN HIROSHIMA UNIV,SCH MED,DEPT BIOCHEM HIROSHIMA 734 JAPAN KAZUSA DNA RES INST KISARAZU 292 JAPAN NATL INST INFECT DIS,DEPT VIROL 2 TOKYO 162 JAPAN KIRIN BREWERY CO LTD,CENT LABS KEY TECHNOL YOKOHAMA KANAGAWA 236 JAPAN
Titolo Testata:
The Journal of biological chemistry
fascicolo: 1, volume: 273, anno: 1998,
pagine: 291 - 295
SICI:
0021-9258(1998)273:1<291:P(RPIA>2.0.ZU;2-#
Fonte:
ISI
Lingua:
ENG
Soggetto:
WISKOTT-ALDRICH SYNDROME; RHO-FAMILY GTPASES; BINDING PROTEIN; CDC42HS; ACTIN; KINASE; EFFECTOR; IDENTIFICATION; ACTIVATION; CALMODULIN;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
32
Recensione:
Indirizzi per estratti:
Citazione:
K. Kobayashi et al., "P140SRA-1 (SPECIFICALLY RAC1-ASSOCIATED PROTEIN) IS A NOVEL SPECIFIC TARGET FOR RAC1 SMALL GTPASE", The Journal of biological chemistry, 273(1), 1998, pp. 291-295

Abstract

Rac1 small GTPase plays pivotal roles in various cell functions such as cell morphology, cell polarity, and cell proliferation, We have previously identified IQGAP1 from bovine brain cytosol as a target for Rad by an affinity purification method, By using the same method, we purified a specifically Rac1-associated protein with a molecular mass of about 140 kDa (p140) from bovine brain cytosol, This protein interacted with guanosine 5'-(3-O-thio)triphosphate (GTP gamma S).glutathione S-transferase (GST)-Rac1 but not with the GDP.GST-Rac1, GTP gamma S.GST-Cdc42, or GTP gamma S.GST-RhoA. The amino acid sequences of this protein revealed that p140 is identified as a product of KIAA0068 gene, Wedenoted this protein as Sra-1 (Specifically Rad-associated protein), Recombinant Sra-1 interacted with GTP gamma S.GST-Rac1 and weakly withGDP.Rac1 but not with GST-Cdc42 or GST-RhoA, The N-terminal domain ofSra-1 (1-407 amino acids) was responsible for the interaction with Rad. Myc-tagged Sra-1 and the deletion mutant capable of interacting with Rad, but not the mutants unable to bind Rad, were colocalized with dominant active Rac1(Val-12) and cortical actin filament at the Rac1(Val-12)-induced membrane ruffling area in KB cells, Sra-1 was cosedimented with filamentous actin (F-actin), indicating that Sra-1 directly interacts with F-actin, These results suggest that Sra-1 is a novel and specific target for Rac1.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 26/01/21 alle ore 03:12:34