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Titolo:
DIFFERENTIAL DISTRIBUTION OF SYNAPSIN IIA AND IIB MESSENGER-RNAS IN VARIOUS BRAIN STRUCTURES AND THE EFFECT OF CHRONIC MORPHINE ADMINISTRATION ON THE REGIONAL EXPRESSION OF THESE ISOFORMS
Autore:
MATUSLEIBOVITCH N; NEVO I; VOGEL Z;
Indirizzi:
WEIZMANN INST SCI,DEPT NEUROBIOL IL-76100 REHOVOT ISRAEL WEIZMANN INST SCI,DEPT NEUROBIOL IL-76100 REHOVOT ISRAEL
Titolo Testata:
Molecular brain research
fascicolo: 2, volume: 45, anno: 1997,
pagine: 301 - 316
SICI:
0169-328X(1997)45:2<301:DDOSIA>2.0.ZU;2-5
Fonte:
ISI
Lingua:
ENG
Soggetto:
RAT SPINAL-CORD; DORSAL-ROOT GANGLION; SYNAPTIC VESICLE PHOSPHOPROTEINS; ADENYLATE-CYCLASE ACTIVITY; ELEMENT-BINDING PROTEIN; OPIATE EXPOSURE LEADS; DELTA-OPIOID RECEPTOR; LOCUS-COERULEUS; SENSORY NEURONS; MESSENGER-RNA;
Keywords:
SYNAPSIN II; OPIATE TOLERANCE; MORPHINE; IN SITU HYBRIDIZATION; REVERSE TRANSCRIPTASE-POLYMERASE CHAIN REACTION (RT-PCR); REGIONAL DISTRIBUTION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
69
Recensione:
Indirizzi per estratti:
Citazione:
N. Matusleibovitch et al., "DIFFERENTIAL DISTRIBUTION OF SYNAPSIN IIA AND IIB MESSENGER-RNAS IN VARIOUS BRAIN STRUCTURES AND THE EFFECT OF CHRONIC MORPHINE ADMINISTRATION ON THE REGIONAL EXPRESSION OF THESE ISOFORMS", Molecular brain research, 45(2), 1997, pp. 301-316

Abstract

Quantitative reverse transcriptase-polymerase chain reaction and in situ hybridization techniques were used to determine the regional distribution of synapsin IIa and IIb mRNAs in rat central nervous system and to assess the effect of chronic morphine administration on the gene expression of these two isoforms of synapsin II. These isoforms are members of a family of neuron-specific phosphoproteins thought to be involved in the regulation of neurotransmitter release. Our data demonstrate the widespread distribution, yet regionally variable expression, of synapsin IIa and IIb mRNAs throughout the adult rat brain and spinalcord. The ratios of the relative abundance of synapsins IIa and IIb differed by up to 4.5-fold among the various regions studied. Synapsin IIa and IIb mRNAs were shown to be highly concentrated in the thalamusand in the hippocampus, whereas lower concentrations were found in most other central nervous system structures. In this study, we show differential regulation by morphine of synapsins IIa and IIb in various regions of the brain. In the striatum, a 2.4-fold increase was observedin the levels of synapsin IIa mRNA following chronic morphine regime,whereas no change was found for synapsin IIb. On the other hand, mRNAlevels of synapsin IIb in spinal cord of chronically treated rats were markedly decreased (by 62%), while no alterations were observed in synapsin IIa. Selective regulation by morphine has also been demonstrated in several other central nervous system structures. The opiate-induced regulation of the gene expression of synapsin II isoforms could beviewed as one of the cellular adaptations to the persistent opiate effects and may be involved in the molecular mechanism underlying opiatetolerance and/or dependence.

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Documento generato il 04/07/20 alle ore 12:20:11