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Titolo:
ANALYSIS OF THE MECHANISM(S) OF METAPHASE-I ARREST IN STRAIN LT MOUSEOOCYTES - PARTICIPATION OF MOS
Autore:
HIRAO Y; EPPIG JJ;
Indirizzi:
JACKSON LAB,600 MAIN ST BAR HARBOR ME 04609 JACKSON LAB BAR HARBOR ME 04609
Titolo Testata:
Development
fascicolo: 24, volume: 124, anno: 1997,
pagine: 5107 - 5113
SICI:
0950-1991(1997)124:24<5107:AOTMOM>2.0.ZU;2-A
Fonte:
ISI
Lingua:
ENG
Soggetto:
MATURATION-PROMOTING FACTOR; ACTIVATED PROTEIN-KINASES; MAP KINASE; MEIOTIC METAPHASE; CELL-CYCLES; PARTHENOGENETIC DEVELOPMENT; SECONDARY OOCYTES; XENOPUS-OOCYTES; GERM-CELLS; MICE;
Keywords:
OOCYTE; METAPHASE I; MEIOSIS; MOUSE; CYTOSTATIC FACTOR; MOS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
46
Recensione:
Indirizzi per estratti:
Citazione:
Y. Hirao e J.J. Eppig, "ANALYSIS OF THE MECHANISM(S) OF METAPHASE-I ARREST IN STRAIN LT MOUSEOOCYTES - PARTICIPATION OF MOS", Development, 124(24), 1997, pp. 5107-5113

Abstract

Oocytes of almost all vertebrates become arrested at metaphase II to await fertilization. Arrest is achieved with the participation of a protein complex known as cytostatic factor (CSF) that stabilizes histoneH1 kinase activity. MOS and mitogen-activated protein kinase (MAPK) are important components of CSF, Strain LT/Sv mice, and strains relatedto LT/Sv, produce a high percentage of atypical oocytes that are arrested at metaphase I when normal oocytes have progressed to metaphase II. The potential role of MOS in metaphase I arrest was investigated using strain LT/Sv and LT-related recombinant inbred strains, LTXBO and CX8-4. MOS and MAPK are produced and functional in maturing LT oocytes, Two experimental paradigms were used to reduce or delete MOS in LT oocytes and assess effects on metaphase I arrest. First, sense and antisense Mos oligonucleotides were microinjected into metaphase I-arrested oocytes, Antisense, but not sense, Mos oligonucleotides promoted theactivation of metaphase I-arrested oocytes. Second, mice carrying a Mos null mutation were crossed with LT mice, the null mutation was backcrossed three times to LT mice, and Mos(+/-) N-3 mice were intercrossed to produce Mos(-/-), Mos(+/-) and Mos(+/+) N3F1 mice. Oocytes of allthree Mos genotypes of N3F1 mice sustained meiotic arrest for 17 hours indicating that metaphase I arrest is not initiated by a MOS-dependent mechanism. However, unlike Mos(+/+) and Mos(+/-) CX8-4 N3F1 oocytes. metaphase I arrest of Mos(-/-) CX8-4 N3F1 oocytes was not sustained after 17 hours and became reversed gradually. These results, like the antisense Mos oligonucleotide microinjection experiments, suggest thatMOS participates in sustaining metaphase I arrest in LT oocytes.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 24/09/20 alle ore 08:18:10