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Titolo:
LOCALIZATION OF PEPTIDE TRANSPORTER IN NUCLEI AND LYSOSOMES OF THE PANCREAS
Autore:
BOCKMAN DE; GANAPATHY V; OBLAK TG; LEIBACH FH;
Indirizzi:
MED COLL GEORGIA,DEPT CELLULAR BIOL & ANAT AUGUSTA GA 30912 MED COLL GEORGIA,DEPT BIOCHEM & MOL BIOL AUGUSTA GA 30912
Titolo Testata:
International journal of pancreatology
fascicolo: 3, volume: 22, anno: 1997,
pagine: 221 - 225
SICI:
0169-4197(1997)22:3<221:LOPTIN>2.0.ZU;2-J
Fonte:
ISI
Lingua:
ENG
Soggetto:
BORDER MEMBRANE-VESICLES; DIPEPTIDE TRANSPORT; EXPRESSION CLONING; RABBIT; INTESTINE;
Keywords:
PEPTIDE TRANSPORTER; PEPT 1; SMOOTH MUSCLE CELL NUCLEI; ACINAR CELL LYSOSOMES; SCHWANN CELL NUCLEI;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
17
Recensione:
Indirizzi per estratti:
Citazione:
D.E. Bockman et al., "LOCALIZATION OF PEPTIDE TRANSPORTER IN NUCLEI AND LYSOSOMES OF THE PANCREAS", International journal of pancreatology, 22(3), 1997, pp. 221-225

Abstract

Conclusions. These studies show for the first time the localization of a H+/peptide cotransporter in nuclei of vascular smooth muscle cellsand Schwann cells and its localization in lysosomes of the exocrine pancreas. It is likely that the transporter functions to move small peptides from the lysosome to the cytoplasm following intralysosomal protein degradation. The nature of the transporter function in the nucleusremains to be determined, including the possibility that peptide signaling molecules may be transmitted between nucleus and cytoplasm, Background. PEPT1 transports di-and tripeptides through plasma membranes. Peptides are cotransported with H+, thus deriving the energy for the active transport process from an electrochemical H+ gradient. The main regions in which PEPT1 has been thought to function are the plasma membranes of the small intestinal epithelial cells for absorption of protein digestion products and in the kidney tubules for recovery of smallpeptides from the glomerular filtrate. Methods. Pancreas was removed from rats and quick frozen with liquid nitrogen. Frozen sections were fixed in cold acetone. Sections were incubated with primary antibody against PEPT1, followed by a secondary antibody conjugated with fluorescein, then examined with a fluorescence microscope. Results, Three major structures were immunopositive with the antibody to PEPT1: the nuclei of smooth muscle cells in the wall of arterioles, the nuclei of Schwann cells in unmyelinated pancreatic nerves, and lysosomes in acinar cells.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 02/04/20 alle ore 19:26:51