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Titolo:
A MURINE MONOCLONAL-ANTIBODY(928) RECOGNIZING A NEW EPITOPE FORMED WITH A COMBINATION OF HLA-DPA1-ASTERISK-0201 AND DPB1-ASTERISK-0301 GENE-PRODUCTS
Autore:
KISHI H; OKUMURA A; TONG JJ; SUGIYAMA E; MATSUNO H; MINOWADA J; KANAI T; NISHIMURA Y; MURAGUCHI A;
Indirizzi:
TOYAMA MED & PHARMACEUT UNIV,FAC MED,DEPT IMMUNOL,2630 SUGITANI TOYAMA 93001 JAPAN TOYAMA MED & PHARMACEUT UNIV,FAC MED,DEPT IMMUNOL TOYAMA 93001 JAPAN TOYAMA MED & PHARMACEUT UNIV,FAC MED,DEPT INTERNAL MED 1 TOYAMA 93001JAPAN TOYAMA MED & PHARMACEUT UNIV,FAC MED,DEPT ORTHOPED TOYAMA 93001 JAPAN HAYASHIBARA BIOCHEM LABS INC,FUJISAKI CELL CTR OKAYAMA 702 JAPAN KUMAMOTO UNIV,GRAD SCH MED SCI,DEPT NEUROSCI & IMMUNOL,DIV IMMUNOGENET KUMAMOTO 860 JAPAN
Titolo Testata:
Human immunology
fascicolo: 1-2, volume: 56, anno: 1997,
pagine: 114 - 124
SICI:
0198-8859(1997)56:1-2<114:AMMRAN>2.0.ZU;2-Z
Fonte:
ISI
Lingua:
ENG
Soggetto:
MHC CLASS-II; HLA-DR; CELL-LINES; SURFACE EXPRESSION; INVARIANT CHAIN; HUMAN MONOCYTES; IFN-GAMMA; T-CELLS; B-CELLS; ANTIGEN;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
42
Recensione:
Indirizzi per estratti:
Citazione:
H. Kishi et al., "A MURINE MONOCLONAL-ANTIBODY(928) RECOGNIZING A NEW EPITOPE FORMED WITH A COMBINATION OF HLA-DPA1-ASTERISK-0201 AND DPB1-ASTERISK-0301 GENE-PRODUCTS", Human immunology, 56(1-2), 1997, pp. 114-124

Abstract

A murine monoclonal antibody (mAb), 928, that recognizes a cell surface antigen (928 Ag) on a human Epstein-Barr virus-transformed fetal liver-derived lymphoid progenitor cell line (FL4.4) was generated. The 928 mAb reacted with only FL4.4; it did not react with any other 57 cell lines tested. Two color flowcytometry analysis of peripheral blood mononuclear cells (PBMC) revealed thar the 925 mAb reacted with B cell and monocyte fractions from only two individuals out of 63 unrelated donors. Biochemical analyses showed that the 928 Ag composes of two molecules (33 and 34 K-d) and forms a SDS-resistant, noncovalently linkeddimer conformation, the feature being similar to that of peptide-bound MHC class II molecules. Treatment of FL4.4 cells with the 925 mAb significantly facilitated homotypic cell aggregation. In addition, treatment of PBMC of the 928 Ag+ donor with recombinant IL-4 augmented the expression of the 928 Ag on CD64(+) monocytes. Typing of HLA-DRB1, DPA1 and DPB1 alleles of the 925 Ag expressing and nonexpressing cells revealed that the 328 Ag is expressed only on PBMC of HLA-DPA0201 and DPB10301 positive donors. Finally, anti-DP antibody precleared 928 Ag from the cell lysate. These results demonstrate that the 328 mAb recognizes a polymorphic determinant of HLA-DPA10201-DPB1*0301 gene produces. The possibility that amino acids in the groove of the peptide-binding site of HLA-DP molecules are critical for the 928 epitope is discussed. American Society for Histocompatibility and Immunogenetics, 1997. Published by Elsevier Science Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/12/20 alle ore 06:30:02