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Titolo:
HUMAN ACELLULAR DERMAL MATRIX AS A NOVEL MODEL OF MALIGNANT EPITHELIAL-CELL INVASION
Autore:
BULLARD KM; BANDA MJ; ARBEIT JM; BERGSLAND E; YOUNG DM;
Indirizzi:
SAN FRANCISCO GEN HOSP 0807,DEPT SURG,1001 POTRERO AVE SAN FRANCISCO CA 94110 UNIV CALIF SAN FRANCISCO,DEPT SURG SAN FRANCISCO CA 94143 UNIV CALIF SAN FRANCISCO,RADIOBIOL LAB SAN FRANCISCO CA 94143 UNIV CALIF SAN FRANCISCO,HORMONE RES INST SAN FRANCISCO CA 94143
Titolo Testata:
Invasion & metastasis
fascicolo: 1, volume: 17, anno: 1997,
pagine: 42 - 52
SICI:
0251-1789(1997)17:1<42:HADMAA>2.0.ZU;2-X
Fonte:
ISI
Lingua:
ENG
Soggetto:
RECONSTITUTED BASEMENT-MEMBRANE; IV COLLAGENASE; TUMOR-CELLS; INTERSTITIAL COLLAGENASE; CONNECTIVE-TISSUE; TRANSGENIC MICE; INVITRO ASSAY; HUMAN SKIN; GROWTH; METALLOPROTEINASES;
Keywords:
TUMOR INVASION; ACELLULAR DERMIS; COLLAGENASE; MATRIX METALLOPROTEINASES;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
32
Recensione:
Indirizzi per estratti:
Citazione:
K.M. Bullard et al., "HUMAN ACELLULAR DERMAL MATRIX AS A NOVEL MODEL OF MALIGNANT EPITHELIAL-CELL INVASION", Invasion & metastasis, 17(1), 1997, pp. 42-52

Abstract

Cancer invasion and metastasis are associated with matrix degradation, We describe a novel in vivo model of invasion by squamous epithelialneoplastic cells derived from transgenic mice grown on acellular human dermis. Human dermis was subjected to multiple freeze-thaw cycles torender it acellular, maintaining the basement membrane of the former dermal-epidermal junction, Cells representing discrete stages of a multistep transgenic mouse model of epidermal carcinogenesis (neonatal transgenic keratinocytes, moderately/poorly differentiated squamous cellcarcinoma, and lymph node metastasis) were seeded onto the basement membrane surface, grown in culture for 4 days, grafted in a subpannicular pocket of athymic mice, and harvested after 3 weeks, Histological analysis demonstrated that neonatal transgenic keratinocytes did not degrade the basement membrane or invade the underlying dermis, In contrast, malignant cells derived from both a moderately differentiated squamous carcinoma and a lymph node metastasis were highly invasive, Immunohistochemical analysis revealed collagenase only in nests of invadingmalignant cells in contact with the dermal. matrix, but not in the tumor mass remaining above the basement membrane, suggesting that this proteinase may be required for stromal invasion, This novel model recapitulates the events seen in malignant invasion: transgenic keratinocytes are unable to penetrate the dermis while cells from a moderately differentiated carcinoma and from lymph node metastasis consistently invade.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 25/11/20 alle ore 15:46:32