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Titolo:
INTERACTION OF CU(II) 3,5-DIISOPROPYLSALICYLATE WITH HUMAN SERUM-ALBUMIN - AN EVALUATION OF SPECTROSCOPIC DATA
Autore:
GREENAWAY FT; HAHN JJ; XI N; SORENSON JRJ;
Indirizzi:
CLARK UNIV,GUSTAF H CARLSON SCH CHEM WORCESTER MA 01610 CLARK UNIV,GUSTAF H CARLSON SCH CHEM WORCESTER MA 01610 COLL PHARM,DEPT BIOPHARMACEUT SCI,DIV MED CHEM LITTLE ROCK AR 72205
Titolo Testata:
BioMetals
fascicolo: 1, volume: 11, anno: 1998,
pagine: 21 - 26
SICI:
0966-0844(1998)11:1<21:IOC3WH>2.0.ZU;2-V
Fonte:
ISI
Lingua:
ENG
Soggetto:
COPPER(II) COMPLEXES; BLOOD-PLASMA; BINDING; SITE; RESONANCE; DOG; INVOLVEMENT; CONSTANTS; ZINC(II); PROTEINS;
Keywords:
COPPER(II) 3,5-DIISOPROPYLSALICYLATE; EPR; HUMAN SERUM ALBUMIN; NMR;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
30
Recensione:
Indirizzi per estratti:
Citazione:
F.T. Greenaway et al., "INTERACTION OF CU(II) 3,5-DIISOPROPYLSALICYLATE WITH HUMAN SERUM-ALBUMIN - AN EVALUATION OF SPECTROSCOPIC DATA", BioMetals, 11(1), 1998, pp. 21-26

Abstract

The copper(II) complex of 3,5-diisopropylsalicylate is a lipophilic water-insoluble binuclear complex, Cu(II)(2)(3,5-DIPS)(4), that has attracted interest because of a wide range of pharmacological activities. This study was undertaken to examine bonding interactions between thecomplex and human serum albumin (HSA) to help elucidate the mode of transport of the complex in vivo. Electron paramagnetic resonance, numerical magnetic resonance and UV-visible absorption spectroscopic studies were performed using 200 mu M aqueous solutions (pH 7.5) of HSA to which had been added up to three molar equivalents of CuCl2, CuSO4, orCu(II)(2)(3,5-DIPS)(4). Both EPR and UV-visible spectra demonstrated the presence of more than one copper bonding site on HSA, and proton NMR spectra showed that the 3,5-DIPS ligand is also bonded to HSA. These results indicate that there is no observable direct coordination of the ligand to copper in the presence of HSA, and that the majority of the copper and 3,5-DIPS bond to HSA at separate sites. Addition of solid Cu(II)(2)(3,5-DIPS)(4) to HSA at pH 7.5 similarly resulted in spectra that suggest that there are no ternary Cu(II)(3,5-DIPS), Cu(II)(3,5-DIPS)(2), or Cu(II)(2)(3,5-DIPS)(4) complexes formed with HSA. It is concluded that any ternary complexes formed in the presence of HSA arebelow the spectroscopic detection limits and represent less than 5% of the total copper.

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Documento generato il 28/11/20 alle ore 21:45:45