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Titolo:
DISSECTION OF C1Q CAPABILITY OF INTERACTING WITH IGG - TIME-DEPENDENTFORMATION OF A TIGHT AND ONLY PARTLY REVERSIBLE ASSOCIATION
Autore:
KAUL M; LOOS M;
Indirizzi:
UNIV MAINZ,INST MED MICROBIOL & HYG,HOCHHAUS AUGUSTUSPL D-55101 MAINZGERMANY UNIV MAINZ,INST MED MICROBIOL & HYG,HOCHHAUS AUGUSTUSPL D-55101 MAINZGERMANY
Titolo Testata:
The Journal of biological chemistry
fascicolo: 52, volume: 272, anno: 1997,
pagine: 33234 - 33244
SICI:
0021-9258(1997)272:52<33234:DOCCOI>2.0.ZU;2-7
Fonte:
ISI
Lingua:
ENG
Soggetto:
1ST COMPONENT; MONOCLONAL-ANTIBODIES; COMPLEMENT COMPONENT; IMMUNOGLOBULIN-G; ELECTRON-MICROSCOPY; SUBCOMPONENT C1Q; MEMBRANE-PROTEIN; BINDING-SITE; HUMAN CLQ; MACROPHAGES;
Tipo documento:
Article
Natura:
Periodico
Citazioni:
39
Recensione:
Indirizzi per estratti:
Citazione:
M. Kaul e M. Loos, "DISSECTION OF C1Q CAPABILITY OF INTERACTING WITH IGG - TIME-DEPENDENTFORMATION OF A TIGHT AND ONLY PARTLY REVERSIBLE ASSOCIATION", The Journal of biological chemistry, 272(52), 1997, pp. 33234-33244

Abstract

C1q-bearing immune complexes have been observed in diseases such as rheumatoid arthritis and human immunodeficiency virus infection-associated neuropathy. For the purpose of understanding better the phenomenonof C1q-bearing immune complexes, we investigated the constancy of theC1q-IgG interaction. An enzyme-linked immunosorbent assay was developed in which wells were coated with IgG to mimic antigen-complexed IgG. Serial dilutions of C1q were applied for distinct time intervals, andbound C1q was detected either directly or after exposure to one of several elution buffers. Our results show that a part of C1q attached toIgG forms a tight association that is not reversible under treatment with buffers containing usually protein-protein interaction-dissociating reagents such as 3 M NaCl, 5 M urea, sodium dodecyl sulfate, or beta-mercaptoethanol. The formation of the highly stable C1q-IgG complex was found to be time-, temperature-, and pH-dependent and to proceed with bound C1q even in the absence of free C1q in the supernatant. In ligand blotting experiments we demonstrate for the first time directly that all three chains of C1q can individually bind IgG. Altogether, our results provide a suitable explanation for the formation and persistence of C1q-bearing immune complexes.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 08/07/20 alle ore 00:53:25