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Titolo:
A CASPASE-ACTIVATED DNASE THAT DEGRADES DNA DURING APOPTOSIS, AND ITSINHIBITOR ICAD
Autore:
ENARI M; SAKAHIRA H; YOKOYAMA H; OKAWA K; IWAMATSU A; NAGATA S;
Indirizzi:
OSAKA UNIV,SCH MED,DEPT GENET,2-2 YAMADA OKA OSAKA 565 JAPAN OSAKA UNIV,SCH MED,DEPT GENET OSAKA 565 JAPAN KIRIN BREWERY CO LTD,CENT LAB KEY TECHNOL KANAGAWA 236 JAPAN OSAKA BIOSCI INST OSAKA 565 JAPAN
Titolo Testata:
Nature
fascicolo: 6662, volume: 391, anno: 1998,
pagine: 43 - 50
SICI:
0028-0836(1998)391:6662<43:ACDTDD>2.0.ZU;2-C
Fonte:
ISI
Lingua:
ENG
Soggetto:
FAS-MEDIATED APOPTOSIS; NF-KAPPA-B; CELL-DEATH; ICE-LIKE; PROTEASE; PROTEINS; FAMILY; FRAGMENTATION; IDENTIFICATION; INVOLVEMENT;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
44
Recensione:
Indirizzi per estratti:
Citazione:
M. Enari et al., "A CASPASE-ACTIVATED DNASE THAT DEGRADES DNA DURING APOPTOSIS, AND ITSINHIBITOR ICAD", Nature, 391(6662), 1998, pp. 43-50

Abstract

The homeostasis of animals is regulated not only by the growth and differentiation of cells, but also by cell death through a process knownas apoptosis. Apoptosis is mediated by members of the caspase family of proteases, and eventually causes the degradation of chromosomal DNA. A caspase-activated deoxyribonuclease (CAD) and its inhibitor (ICAD)have now been identified in the cytoplasmic fraction of mouse lymphoma cells. CAD is a protein of 343 amino acids which carries a nuclear-localization signal; ICAD exists in a long and a short form. Recombinant ICAD specifically inhibits CAD-induced degradation of nuclear DMA and its DNase activity. When CAD is expressed with ICAD In COS cells or in a cell-free system, CAD is produced as a complex with ICAD: treatment with caspase 3 releases the DNase activity which causes DNA fragmentation in nuclei. ICAD therefore seems to function as a chaperone for CAD during its synthesis, remaining complexed with CAD to inhibit its DNase activity; caspases activated by apoptotic stimuli then cleave ICAD, allowing CAD to enter the nucleus and degrade chromosomal DNA.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 21/01/21 alle ore 02:48:22