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Titolo:
P2Z PURINOCEPTOR-ASSOCIATED PORES INDUCED BY EXTRACELLULAR ATP IN MACROPHAGES AND J774 CELLS
Autore:
COUTINHOSILVA R; PERSECHINI PM;
Indirizzi:
FED UNIV RIO DE JANEIRO,INST BIOFIS CARLOS CHAGAS FILHO,LAB IMUNOBIOFIS,BLOCO G CCS,ILHA FUNDAO BR-21941900 RIO JANEIRO BRAZIL FED UNIV RIO DE JANEIRO,INST BIOFIS CARLOS CHAGAS FILHO,LAB IMUNOBIOFIS BR-21941900 RIO JANEIRO BRAZIL
Titolo Testata:
American journal of physiology. Cell physiology
fascicolo: 6, volume: 42, anno: 1997,
pagine: 1793 - 1800
SICI:
0363-6143(1997)42:6<1793:PPPIBE>2.0.ZU;2-4
Fonte:
ISI
Lingua:
ENG
Soggetto:
P-2Z PURINERGIC RECEPTORS; MOUSE MACROPHAGES; PLASMA-MEMBRANES; PHAGOCYTIC-CELLS; THYMIC RETICULUM; FORMING PROTEIN; MAST-CELLS; ION FLUXES; CHANNELS; INHIBITION;
Keywords:
ADENOSINE 5'-TRIPHOSPHATE; PERMEABILIZATION; 3'-O-(4-BENZOYLBENZOYL)-ADENOSINE 5'-TRIPHOSPHATE; OXIDIZED ADENOSINE 5'-TRIPHOSPHATE; URIDINE 5'-TRIPHOSPHATE; P2X(7);
Tipo documento:
Article
Natura:
Periodico
Citazioni:
39
Recensione:
Indirizzi per estratti:
Citazione:
R. Coutinhosilva e P.M. Persechini, "P2Z PURINOCEPTOR-ASSOCIATED PORES INDUCED BY EXTRACELLULAR ATP IN MACROPHAGES AND J774 CELLS", American journal of physiology. Cell physiology, 42(6), 1997, pp. 1793-1800

Abstract

Millimolar concentrations of extracellular ATP (ATP(o)) can induce the permeabilization of plasma membranes of macrophages and other bone marrow-derived cells to low-molecular-weight solutes, a phenomenon thatis the hallmark of P2Z purinoceptors. However, patch-clamp and whole cell electrophysiological experiments have so far failed to demonstrate the existence of any ATP(o)-induced P2Z-associated pores underlying this permeabilization phenomenon. Here, we describe ATP(o)-induced pores of 409 +/- 33 pS recorded using cell-attached patch-clamp experiments performed in macrophages and J774 cells. These pores are voltage dependent and display several properties of the P2Z-associated permeabilization phenomenon: they are permeable to both large cations and anions, such as tris(hydroxymethyl)aminomethane, N-methyl-D-glucamine, and glutamate; their opening is favored at temperatures higher than 30 degrees C; they are blocked by oxidized ATP and Mg2+; and they can be triggered by 3'-O-(4-benzoylbenzoyl)-ATP but not by UTP or ADP. We conclude that the pores described in this report are associated with the P2Zpermeabilization phenomenon.

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Documento generato il 28/11/20 alle ore 09:32:41