Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
A SIMPLE AND RAPID METHOD FOR THE ANALYSIS OF WATER-SOLUBLE CARBOHYDRATES FROM SMALL SEGMENTS OF CEREAL LEAF TISSUE
Autore:
HARRISON J; GALLAGHER JA; POLLOCK CJ;
Indirizzi:
INST GRASSLAND & ENVIRONM RES,DEPT ENVIRONM BIOL,PLAS GOGERDDAN CEREDIGION SY23 3EB WALES
Titolo Testata:
Journal of plant physiology
fascicolo: 6, volume: 151, anno: 1997,
pagine: 654 - 659
SICI:
0176-1617(1997)151:6<654:ASARMF>2.0.ZU;2-M
Fonte:
ISI
Lingua:
ENG
Soggetto:
LOLIUM-TEMULENTUM L; SUCROSE PHOSPHORYLASE; FRUCTAN BIOSYNTHESIS; EXCISED LEAVES; ASSAY;
Keywords:
PLANTS; CARBOHYDRATES; GLUCOSE; FRUCTOSE; SUCROSE; FRUCTAN; LEAVES;
Tipo documento:
Article
Natura:
Periodico
Citazioni:
13
Recensione:
Indirizzi per estratti:
Citazione:
J. Harrison et al., "A SIMPLE AND RAPID METHOD FOR THE ANALYSIS OF WATER-SOLUBLE CARBOHYDRATES FROM SMALL SEGMENTS OF CEREAL LEAF TISSUE", Journal of plant physiology, 151(6), 1997, pp. 654-659

Abstract

A simple three-step assay has been developed for the analysis of nanomole quantities of glucose, fructose and sucrose from small plant samples in the presence of-fructans. The assay of crude ethanol/water extracts proceeds by the sequential addition of the commercially availableenzymes; hexokinase, phosphogluco-isomerase, and sucrose phosphorylase. The assay was compared with conventional coupled assays for glucoseand fructose, together with TLC and HPLC assays for total water-soluble carbohydrates. AU assays showed similar results over the range of standards and plant samples tested. The assay has been developed for use on microtiter plate readers, allowing for the screening of-large numbers of samples rapidly. Fructan content in the same plant samples canbe calculated following the determination of total free and combined hexoses.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 01/12/20 alle ore 16:06:50