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Titolo:
ANTIOXIDATIVE PROTECTION IN A HIGH-MELATONIN ORGANISM - THE DINOFLAGELLATE GONYAULAX-POLYEDRA IS RESCUED FROM LETHAL OXIDATIVE STRESS BY STRONGLY ELEVATED, BUT PHYSIOLOGICALLY POSSIBLE CONCENTRATIONS OF MELATONIN
Autore:
ANTOLIN I; OBST B; BURKHARDT S; HARDELAND R;
Indirizzi:
UNIV GOTTINGEN,INST ZOOL 1,BERLINER STR 28 D-37073 GOTTINGEN GERMANY UNIV GOTTINGEN,INST ZOOL 1 D-37073 GOTTINGEN GERMANY UNIV OVIEDO,DEPT MORFOL & BIOL CELULAR E-33006 OVIEDO ASTURIAS SPAIN
Titolo Testata:
Journal of pineal research
fascicolo: 4, volume: 23, anno: 1997,
pagine: 182 - 190
SICI:
0742-3098(1997)23:4<182:APIAHO>2.0.ZU;2-3
Fonte:
ISI
Lingua:
ENG
Soggetto:
GLUTATHIONE-PEROXIDASE ACTIVITY; NEUROHORMONE MELATONIN; SUPEROXIDE-DISMUTASE; LIPID-PEROXIDATION; IN-VIVO; BIOLUMINESCENCE; DAMAGE; RATS; INDOLEAMINES; STIMULATION;
Keywords:
DINOFLAGELLATE; FREE RADICALS; GONYAULAX; MELATONIN; OXIDATIVE STRESS;
Tipo documento:
Article
Natura:
Periodico
Citazioni:
59
Recensione:
Indirizzi per estratti:
Citazione:
I. Antolin et al., "ANTIOXIDATIVE PROTECTION IN A HIGH-MELATONIN ORGANISM - THE DINOFLAGELLATE GONYAULAX-POLYEDRA IS RESCUED FROM LETHAL OXIDATIVE STRESS BY STRONGLY ELEVATED, BUT PHYSIOLOGICALLY POSSIBLE CONCENTRATIONS OF MELATONIN", Journal of pineal research, 23(4), 1997, pp. 182-190

Abstract

Lethal oxidative stress was investigated in the dinoflagellate Gonyaulax polyedra by measuring the dying-peak of bioluminescence during circadian phases of low physiological light emission, low bioluminescencecapacity, and low sensitivity to stimulatory agents. Measurements were carried out in constant darkness after transfer of cells from light at CT 6 (circadian time, 0600 hr). H2O2 (0.08 mM), when administered 1hr after transfer of cells, led to a multifold, long-lasting enhancement of light emission, which is typical for lethal cell damage. At thecircadian phases of investigation, melatonin did not substantially stimulate bioluminescence up to concentrations of 0.5 mM. At this concentration, addition of melatonin prevented the dying-peak and reduced bioluminescence to almost basal values. The high concentration of melatonin applied is not unphysiological in Gonyaulax, because the indoleamine can increase to levels of several millimolar, e.g., in response to temperature signals. These protective effects of melatonin seem to be caused mainly by the direct action of melatonin as an antioxidant, because the major enzymes of antioxidative protection were not stimulatedby melatonin, although some of them responded to H2O2. The activitiesof neither superoxide dismutase, hemoperoxidase/catalase, glutathioneperoxidase, nor haloperoxidase were enhanced under the influence of melatonin; glutathione S-transferase activity increased only slightly.

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Documento generato il 12/07/20 alle ore 04:29:29