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Titolo:
FURTHER ANALYSIS OF INTERLEUKIN-2 RECEPTOR SUBUNIT EXPRESSION ON THE DIFFERENT HUMAN PERIPHERAL-BLOOD MONONUCLEAR CELL SUBSETS
Autore:
DAVID D; BANI L; MOREAU JL; DEMAISON C; SUN K; SALVUCCI O; NAKARAI T; DEMONTALEMBERT M; CHOUAIB S; JOUSSEMET M; RITZ J; THEZE J;
Indirizzi:
INST PASTEUR,DEPT IMMUNOL,UNITE IMMUNOGENET CELLULAIRE,25 & 28 RUE DRROUX F-75724 PARIS 15 FRANCE INST PASTEUR,DEPT IMMUNOL,UNITE IMMUNOGENET CELLULAIRE F-75724 PARIS 15 FRANCE INST GUSTAVE ROUSSY,IMMUNOL LAB VILLEJUIF FRANCE DANA FARBER CANC INST,DIV HEMATOL MALIGNANCIES BOSTON MA 02115 HOP NECKER ENFANTS MALAD,SITE TRANSFUS PARIS FRANCE CTR TRANSFUS SANGUINE JEAN JULLIARD CLAMART FRANCE
Titolo Testata:
Blood
fascicolo: 1, volume: 91, anno: 1998,
pagine: 165 - 172
SICI:
0006-4971(1998)91:1<165:FAOIRS>2.0.ZU;2-Q
Fonte:
ISI
Lingua:
ENG
Soggetto:
NATURAL-KILLER-CELLS; GAMMA-CHAIN GENE; IL-2 RECEPTOR; T-CELL; HUMAN MONOCYTES; BETA-CHAIN; ALPHA-CHAIN; INTERFERON-GAMMA; ACTIVATION; GROWTH;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
47
Recensione:
Indirizzi per estratti:
Citazione:
D. David et al., "FURTHER ANALYSIS OF INTERLEUKIN-2 RECEPTOR SUBUNIT EXPRESSION ON THE DIFFERENT HUMAN PERIPHERAL-BLOOD MONONUCLEAR CELL SUBSETS", Blood, 91(1), 1998, pp. 165-172

Abstract

We have investigated the expression of the three components of the interleukin-2 receptor (IL-2R alpha, IL-2R beta, and IL-2R gamma) on thesurface of the various peripheral blood mononuclear cell (PBMC) subsets by flow cytometry analysis. The PBMC were immediately isolated (ficoll) from blood collected on heparin as anticoagulant. The three IL-2Rcomponents are absent or only marginally detectable on CD4 T lymphocytes. No expression of the IL-2R chains is found for the B lymphocytes. In most donors, the three chains are not detectable on CD8 T lymphocytes, but for a few of them, IL-2R beta or IL-2R gamma are clearly expressed. CD56 high (IL-2R alpha(+)) and CD56 low (IL-2R alpha(-)) natural kilter (NK) cells express IL-2R beta, but not IL-2R gamma. IL-2R gamma is expressed by monocytes of all donors although with variable intensity. When blood is collected on other anticoagulants or when cells are isolated 1 day after collection, IL-2R alpha, IL-2R beta, and IL-2Rgamma are largely expressed on the surface of most PBMC. This observation provides a possible explanation for divergent data previously reported on IL-2R expression. Finally. we show that IL-2R gamma, which isnot detectable on the cell surface of lymphocytes, is nevertheless expressed and stored as an intracellular component. This result is in agreement with the constitutive expression of the IL-2R gamma gene and suggests a specific regulatory mechanism for IL-2R gamma membrane translocation. (C) 1998 by The American Society of Hematology.

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Documento generato il 22/01/20 alle ore 18:27:24