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Titolo:
IN-VITRO BEHAVIOR OF HEMATOPOIETIC PROGENITOR CELLS UNDER THE INFLUENCE OF CHEMOATTRACTANTS - STROMAL CELL-DERIVED FACTOR-I, STEEL FACTOR, AND THE BONE-MARROW ENVIRONMENT
Autore:
KIM CH; BROXMEYER HE;
Indirizzi:
INDIANA UNIV,SCH MED,DEPT MICROBIOL IMMUNOL,975 W WALNUT ST INDIANAPOLIS IN 46202 INDIANA UNIV,SCH MED,DEPT MICROBIOL IMMUNOL INDIANAPOLIS IN 46202 INDIANA UNIV,SCH MED,DEPT MED INDIANAPOLIS IN 46202 INDIANA UNIV,SCH MED,WALTHER ONCOL CTR INDIANAPOLIS IN 46202 WALTHER CANC INST INDIANAPOLIS IN 00000
Titolo Testata:
Blood
fascicolo: 1, volume: 91, anno: 1998,
pagine: 100 - 110
SICI:
0006-4971(1998)91:1<100:IBOHPC>2.0.ZU;2-M
Fonte:
ISI
Lingua:
ENG
Soggetto:
COLONY-STIMULATING FACTOR; ALDRICH SYNDROME PROTEIN; UMBILICAL-CORD BLOOD; LYMPHOCYTE CHEMOATTRACTANT; MOLECULAR-CLONING; PERIPHERAL-BLOOD; LIGAND; SDF-1; LINE; LESTR/FUSIN;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
39
Recensione:
Indirizzi per estratti:
Citazione:
C.H. Kim e H.E. Broxmeyer, "IN-VITRO BEHAVIOR OF HEMATOPOIETIC PROGENITOR CELLS UNDER THE INFLUENCE OF CHEMOATTRACTANTS - STROMAL CELL-DERIVED FACTOR-I, STEEL FACTOR, AND THE BONE-MARROW ENVIRONMENT", Blood, 91(1), 1998, pp. 100-110

Abstract

How multiple chemoattractants cooperate in directing the migration ofhematopoietic progenitor cells (HPC) for homing and peripheral blood mobilization has not yet been established, We report here the behaviorof HPC under the influence of two different chemoattractants, stromalcell-derived factor (SDF)-1 and steel factor (SLF), and the chemotactic nature of the bone marrow (BM) environment using a two-chamber in vitro migration system. Various formulae were adopted to quantitate these effects. Based on these quantitations, SDF-1 showed only chemotactic activity, while SLF showed both chemotactic and chemokinetic activities on factor-dependent MO7e cells, SLF, like SDF-1, attracted human HPC from a population of CD34(+) cells and induced actin polymerizationin MO7e cells. SLF and SDF-1 cooperated in attracting MO7e cells, as well as cord blood (CB) and BM CD34(+) cells. A negative concentrationgradient of SLF and SDF-1, formed by the presence of chemoattractantsin the upper chamber, showed potent inhibitory effects on MO7e cell migration induced by either of these chemoattractants in the lower chamber, and SDF-1 and SLF were synergistic in mobilizing cells to the lower chamber from this negative chemoattractant gradient, Plasma obtained from BM aspirates, but not CB or peripheral blood, showed strong chemotactic effects on BM and CB CD34(+) cells, and an inhibitory effect in a negative gradient on SDF-1-dependent CD34(+) cell migration, These in vitro migration experiments suggest that chemoattractants such asSDF-1 and SLF with other unidentified BM chemoattractants may be involved cooperatively in the migration of HPC to the BM and in preventingspontaneous mobilization of HPC out of the BM. (C) 1998 by The American Society of Hematology.

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Documento generato il 29/03/20 alle ore 17:57:50