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Titolo:
LACK OF INTERFERON CONSENSUS SEQUENCE BINDING-PROTEIN (ICSBP) TRANSCRIPTS IN HUMAN MYELOID LEUKEMIAS
Autore:
SCHMIDT M; NAGEL S; PROBA J; THIEDE C; RITTER M; WARING JF; ROSENBAUER F; HUHN D; WITTIG B; HORAK I; NEUBAUER A;
Indirizzi:
TECH UNIV DRESDEN,KLIN CARL GUSTAV CARUS,MED KLIN 1,FETSCHERSTR 74 D-01307 DRESDEN GERMANY TECH UNIV DRESDEN,KLIN CARL GUSTAV CARUS,MED KLIN 1 D-01307 DRESDEN GERMANY HUMBOLDT UNIV BERLIN,VIRCHOW KLINIKUM,ABT INNERE MED MS HAMATOL D-1086 BERLIN GERMANY FORSCHUNGSINST MOL PHARMAKOL BERLIN GERMANY FREE UNIV BERLIN,INST MOL BIOL BIOCHEM & BIOINFORMAT D-1000 BERLIN GERMANY
Titolo Testata:
Blood
fascicolo: 1, volume: 91, anno: 1998,
pagine: 22 - 29
SICI:
0006-4971(1998)91:1<22:LOICSB>2.0.ZU;2-Y
Fonte:
ISI
Lingua:
ENG
Soggetto:
CHRONIC MYELOGENOUS LEUKEMIA; REGULATORY FACTOR FAMILY; MARROW STROMAL CELLS; STIMULATED GENES; INDUCIBLE GENES; ALPHA; INDUCTION; GAMMA; EXPRESSION; ELEMENTS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
33
Recensione:
Indirizzi per estratti:
Citazione:
M. Schmidt et al., "LACK OF INTERFERON CONSENSUS SEQUENCE BINDING-PROTEIN (ICSBP) TRANSCRIPTS IN HUMAN MYELOID LEUKEMIAS", Blood, 91(1), 1998, pp. 22-29

Abstract

Interferon consensus sequence binding protein (ICSBP) was first identified as a transcription factor of the interferon (IFN) regulatory factor family (IRF) which regulates expression of IFN-dependent genes by binding to DNA at specific sites, IFN-stimulated responsive elements. Analysis of ICSBP deficient mice showed hematologic alterations similar to chronic myelogenous leukemia (CML) in humans and suggested a novel role for ICSBP in regulating proliferation and differentiation of hematopoietic progenitor cells. Here we show that ICSBP-mRNA expression is impaired in human myeloid leukemias: 27 of 34 CML patients (79%) and 21 of 32 patients with acute myeloid leukemia (AML) (66%) showed very low or absent transcript numbers of ICSBF. In contrast, only 2 of 33normal volunteers (6%) showed low transcription of ICSBP (P < .0001 both for CML and AML values), The lack of expression was not associatedwith lack of lymphatic cells, which normally have been shown to express ICSBP at the highest level. More detailed analysis showed an absence of ICSBP-mRNA also in sorted B cells derived from CML patients, To analyze whether ICSBP may be induced in leukemic cells, ex vivo experiments using a known inducer of ICSBP IFN-gamma, were performed, Ex vivotreatment of primary CML cells using IFN-gamma resulted in induction of ICSBP transcripts. Furthermore, samples of CML patients during IFN-alpha treatment were analyzed, In 11 of 12 CML patients ICSBP-mRNA wasinducible upon in vivo treatment with IFN-alpha, but decreased with progression of CML, Stable transfection of K-562 cell line with ICSBP led to no difference in bcr-abl expression in vitro, although two patients showed an inverse correlation between bcr-abl and ICSBP in vivo, These data suggest that lack of ICSBP may have an important role also in human myeloid leukemogenesis. (C) 1998 by The American Society of Hematology.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/11/20 alle ore 15:52:29